ReviewBiochimica et biophysica acta2012
Detection of ubiquitin-proteasome enzymatic activities in cells: application of activity-based probes to inhibitor development.
Review in Biochimica et biophysica acta, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
- The Role of Deubiquitinating Enzymes in Primary Bone Cancer.Molecular biotechnology · 2025Review
- Activity-Based Protein Profiling (ABPP) for Cellular Deubiquitinase (DUB) and Inhibitor Profiling at Deep and High-Throughput Levels.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Inhibiting UCH-L5: Rational Design of a Cyclic Ubiquitin-Based Peptide Inhibitor.Frontiers in molecular biosciences · 2022Article
- Assay Systems for Profiling Deubiquitinating Activity.International journal of molecular sciences · 2020Review
- Probing enzymatic activity - a radical approach.Chemical science · 2020Article
- Diarylcarbonates are a new class of deubiquitinating enzyme inhibitor.Bioorganic & medicinal chemistry letters · 2019Article
- Comprehensive Landscape of Active Deubiquitinating Enzymes Profiled by Advanced Chemoproteomics.Frontiers in chemistry · 2019Article
- Strategies to Target Specific Components of the Ubiquitin Conjugation/Deconjugation Machinery.Frontiers in chemistry · 2019Review
- Identification of deubiquitinase targets of isothiocyanates using SILAC-assisted quantitative mass spectrometry.Oncotarget · 2017Article
- Activity-based probes for the ubiquitin conjugation-deconjugation machinery: new chemistries, new tools, and new insights.The FEBS journal · 2017Review
- Integrating Pharmacoproteomics into Early-Phase Clinical Development: State-of-the-Art, Challenges, and Recommendations.International journal of molecular sciences · 2017Review
- A cascading activity-based probe sequentially targets E1-E2-E3 ubiquitin enzymes.Nature chemical biology · 2016Article
- Decision for cell fate: deubiquitinating enzymes in cell cycle checkpoint.Cellular and molecular life sciences : CMLS · 2016Review
- Naturally Occurring Isothiocyanates Exert Anticancer Effects by Inhibiting Deubiquitinating Enzymes.Cancer research · 2015Article
- Ubiquitin-Specific Proteases as Druggable Targets.Drug target review · 2015Article
- Screening of DUB activity and specificity by MALDI-TOF mass spectrometry.Nature communications · 2014Article
- Deubiquitinating enzymes as promising drug targets for infectious diseases.Current pharmaceutical design · 2013Review
- Effect of Metabolite Extract ofIranian journal of parasitologyArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
Abstract
backgroundSynthetic probes that mimic natural substrates can enable the detection of enzymatic activities in a cellular environment. One area where such activity-based probes have been applied is the ubiquitin-proteasome pathway, which is emerging as an important therapeutic target. A family of reagents has been developed that specifically label deubiquitylating enzymes (DUBs) and facilitate characterization of their inhibitors. SCOPE OF REVIEW: Here we focus on the application of probes for intracellular DUBs, a group of specific proteases involved in the ubiquitin proteasome system. In particular, the functional characterization of the active subunits of this family of proteases that specifically recognize ubiquitin and ubiquitin-like proteins will be discussed. In addition we present the potential and design of activity-based probes targeting kinases and phosphatases to study phosphorylation. MAJOR
conclusionsSynthetic molecular probes have increased our understanding of the functional role of DUBs in living cells. In addition to the detection of enzymatic activities of known members, activity-based probes have contributed to a number of functional assignments of previously uncharacterized enzymes. This method enables cellular validation of the specificity of small molecule DUB inhibitors. GENERAL SIGNIFICANCE: Molecular probes combined with mass spectrometry-based proteomics and cellular assays represent a powerful approach for discovery and functional validation, a concept that can be expanded to other enzyme classes. This addresses a need for more informative cell-based assays that are required to accelerate the drug development process. This article is part of a Special Issue entitled: Ubiquitin Drug Discovery and Diagnostics.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.