ArticleDevelopmental biology2012
CLASPs function redundantly to regulate astral microtubules in the C. elegans embryo.
Article in Developmental biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 40 citations in OpenAlex.
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- Microtubule stiffening by the doublecortin-domain protein ZYG-8 contributes to mitotic spindle orientation during zygote division in Caenorhabditis elegans.PLoS genetics · 2026Article
- Unveiling inter-embryo variability in spindle length over time: Towards quantitative phenotype analysis.PLoS computational biology · 2024Article
- Interplay Between Polymorphic Short Tandem Repeats and Gene Expression Variation in Caenorhabditis elegans.Molecular biology and evolution · 2023Article
- The large cytoplasmic volume of oocyte.The Journal of reproduction and development · 2023Review
- A modified TurboID approach identifies tissue-specific centriolar components in C. elegans.PLoS genetics · 2022Article
- Differential regulation of single microtubules and bundles by a three-protein module.Nature chemical biology · 2021Article
- The coordination of spindle-positioning forces during the asymmetric division of the Caenorhabditis elegans zygote.EMBO reports · 2021Article
- Orbit/CLASP determines centriole length by antagonising Klp10A inJournal of cell science · 2021Article
- C. elegans CLASP/CLS-2 negatively regulates membrane ingression throughout the oocyte cortex and is required for polar body extrusion.PLoS genetics · 2020Article
- Male meiotic spindle features that efficiently segregate paired and lagging chromosomes.eLife · 2020Article
- Automated phenotyping ofMicrosystems & nanoengineering · 2020Article
- CLASP promotes microtubule bundling in metaphase spindle independently of Ase1/PRC1 in fission yeast.Biology open · 2019Article
- Sumoylation regulates protein dynamics during meiotic chromosome segregation inJournal of cell science · 2019Article
- Microtubule Feedback and LET-99-Dependent Control of Pulling Forces Ensure Robust Spindle Position.Biophysical journal · 2018Article
- Microtubule Dynamics Scale with Cell Size to Set Spindle Length and Assembly Timing.Developmental cell · 2018Article
- New alleles ofmicroPublication biology · 2017Article
- RNAi-Based Functional Genomics Identifies New Virulence Determinants in Mucormycosis.PLoS pathogens · 2017Article
- Cytoplasmic Determination of Meiotic Spindle Size Revealed by a Unique Inter-Species Germinal Vesicle Transfer Model.Scientific reports · 2016Article
- Chromatids segregate without centrosomes during Caenorhabditis elegans mitosis in a Ran- and CLASP-dependent manner.Molecular biology of the cell · 2015Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Microtubule dynamics are thought to play an important role in regulating microtubule interactions with cortical force generating motor proteins that position the spindle during asymmetric cell division. CLASPs are microtubule-associated proteins that have a conserved role in regulating microtubule dynamics in diverse cell types. Caenorhabditis elegans has three CLASP homologs in its genome. CLS-2 is known to localize to kinetochores and is needed for chromosome segregation at meiosis and mitosis; however CLS-1 and CLS-3 have not been reported to have any role in embryonic development. Here, we show that depletion of CLS-2 in combination with either CLS-1 or CLS-3 results in defects in nuclear rotation, maintenance of spindle length, and spindle displacement in the one-cell embryo. Polarity is normal in these embryos, but reduced numbers of astral microtubules reach all regions of the cortex at the time of spindle positioning. Analysis of the microtubule plus-end tracker EB1 also revealed a reduced number of growing microtubules reaching the cortex in CLASP depleted embryos, but the polymerization rate of astral microtubules was not slower than in wild type. These results indicate that C. elegans CLASPs act partially redundantly to regulate astral microtubules and position the spindle during asymmetric cell division. Further, we show that these spindle pole-positioning roles are independent of the CLS-2 binding proteins HCP-1 and HCP-2.
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