ArticleFree radical biology & medicine2012
8-Oxo-2'-deoxyguanosine as a biomarker of tobacco-smoking-induced oxidative stress.
Article in Free radical biology & medicine, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 70 citations in OpenAlex.
- The Effects of Black Raspberry as a Whole Food-Based Approach on Biomarkers of Oxidative Stress in Buccal Cells and Urine of Smokers.Cancer prevention research (Philadelphia, Pa.) · 2024Trial
- The effect of menthol on cigarette smoking behaviors, biomarkers and subjective responses.Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology · 2013Trial
- Smoking accelerates immunosenescence in multiple sclerosis.Frontiers in immunology · 2026Review
- Analyzing 8-Oxoguanine in Exhaled Breath Condensate: A Novel Within-Subject Laboratory Experimental Study on Waterpipe Smokers.Antioxidants (Basel, Switzerland) · 2025Article
- Altered oxidative stress and antioxidant biomarkers concentrations in pregnant individuals exposed to oil and gas sites in Northeastern British Columbia.Toxicological sciences : an official journal of the Society of Toxicology · 2024Article
- Assessment of Oxidative Stress Parameters in Iraqi Male Patients with Covid-19; A Case Control Study.Reports of biochemistry & molecular biology · 2024Article
- Article
- Women suffering from systemic lupus erythematosus are characterized by low blood levels of α-dicarbonyl compounds.Archives of medical science : AMS · 2024Article
- Integrative blood-based characterization of oxidative mitochondrial DNA damage variants implicates Mexican American's metabolic risk for developing Alzheimer's disease.Scientific reports · 2023Article
- Integrative Blood-Based Characterization of Oxidative Mitochondrial DNA Damage Variants Implicates Mexican Americans' Metabolic Risk for Developing Alzheimer's Disease.Research square · 2023Article
- Oxidative stress-related risk of the multiple sclerosis development.Journal of medical biochemistry · 2023Article
- Nitroreduction: A Critical Metabolic Pathway for Drugs, Environmental Pollutants, and Explosives.Chemical research in toxicology · 2022Review
- Article
- Oxidative stress-responsive apoptosis-inducing protein in patients with heterozygous familial hypercholesterolemia.Heart and vessels · 2021Article
- Oxidative stress induction in woodworkers occupationally exposed to wood dust and formaldehyde.Journal of occupational medicine and toxicology (London, England) · 2021Article
- Article
- Development of Low-Cost AuNP-Based Aptasensors with Truncated Aptamer for Highly Sensitive Detection of 8-Oxo-dG in Urine.ACS omega · 2020Article
- Cerebellar gray matter lesions are common in pediatric multiple sclerosis at clinical onset.Journal of neurology · 2020Article
- Lysine-Functionalized Tungsten Disulfide Quantum Dots as Artificial Enzyme Mimics for Oxidative Stress Biomarker Sensing.ACS omega · 2020Article
- Voriconazole enhances UV-induced DNA damage by inhibiting catalase and promoting oxidative stress.Experimental dermatology · 2020Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
7,8-Dihydro-8-oxo-2'-deoxyguanosine (8-oxo-dGuo) is a useful biomarker of oxidative stress. However, its analysis can be challenging because 8-oxo-dGuo must be quantified in the presence of dGuo, without artifactual conversion to 8-oxo-dGuo. Urine is the ideal biological fluid for population studies, because it can be obtained noninvasively and it is less likely that artifactual oxidation of dGuo can occur because of the relatively low amounts that are present compared with hydrolyzed DNA. Stable isotope dilution liquid chromatography-selected reaction monitoring/mass spectrometry (LC-SRM/MS) with 8-oxo-[(15)N(5)]dGuo as internal standard provided the highest possible specificity for 8-oxo-dGuo analysis. Furthermore, artifact formation was determined by addition of [(13)C(10)(15)N(5)]dGuo and monitoring of its conversion to 8-oxo-[(13)C(10)(15)N(5)]dGuo during the analytical procedure. 8-Oxo-dGuo concentrations were normalized for interindividual differences in urine flow by analysis of creatinine using stable isotope dilution LC-SRM/MS. A significant increase in urinary 8-oxo-dGuo was observed in tobacco smokers compared with nonsmokers either using simple urinary concentrations or after normalization for creatinine excretion. The mean levels of 8-oxo-dGuo were 1.65ng/ml and the levels normalized to creatinine were 1.72μg/g creatinine. Therefore, stable isotope dilution LC-SRM/MS analysis of urinary 8-oxo-dGuo complements urinary isoprostane (isoP) analysis for assessing tobacco-smoking-induced oxidative stress. This method will be particularly useful for studies that employ polyunsaturated fatty acids, in which a reduction in arachidonic acid precursor could confound isoP measurements.
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Registered trials
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