ArticleMicrobial biotechnology2012
Light-scattering sensor for real-time identification of Vibrio parahaemolyticus, Vibrio vulnificus and Vibrio cholerae colonies on solid agar plate.
Article in Microbial biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 56 citations in OpenAlex.
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- Identifying antibiotic-resistant strains via cell sorting and elastic-light-scatter phenotyping.Applied microbiology and biotechnology · 2024Article
- Petri-plate, bacteria, and laser optical scattering sensor.Frontiers in cellular and infection microbiology · 2022Review
- Identification of colonies of cultured shellfish-associatedCurrent research in microbial sciences · 2021Article
- Elastic Light Scatter Pattern Analysis for the Expedited Detection ofFrontiers in microbiology · 2021Article
- Advances in Optical Detection of Human-Associated Pathogenic Bacteria.Molecules (Basel, Switzerland) · 2020Review
- Early detection and classification of live bacteria using time-lapse coherent imaging and deep learning.Light, science & applications · 2020Article
- Colony Fingerprint-Based Discrimination ofSensors (Basel, Switzerland) · 2018Article
- Microfluidic multi-angle laser scattering system for rapid and label-free detection of waterborne parasites.Biomedical optics express · 2018Article
- Light Emitting Marker for Robust Vision-Based On-The-Spot Bacterial Growth Detection.Sensors (Basel, Switzerland) · 2017Article
- Colony fingerprint for discrimination of microbial species based on lensless imaging of microcolonies.PloS one · 2017Article
- Virulence Gene-Associated Mutant Bacterial Colonies Generate Differentiating Two-Dimensional Laser Scatter Fingerprints.Applied and environmental microbiology · 2016Article
- Optical scatter patterns facilitate rapid differentiation of Enterobacteriaceae on CHROMagar™ Orientation medium.Microbial biotechnology · 2016Article
- Novel PCR Assays Complement Laser Biosensor-Based Method and Facilitate Listeria Species Detection from Food.Sensors (Basel, Switzerland) · 2015Article
- The pathogenesis, detection, and prevention of Vibrio parahaemolyticus.Frontiers in microbiology · 2015Article
- Streptomycin Induced Stress Response in Salmonella enterica Serovar Typhimurium Shows Distinct Colony Scatter Signature.PloS one · 2015Article
- Article
- Laser-induced speckle scatter patterns in Bacillus colonies.Frontiers in microbiology · 2014Article
- Light scattering sensor for direct identification of colonies of Escherichia coli serogroups O26, O45, O103, O111, O121, O145 and O157.PloS one · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The three most common pathogenic species of Vibrio, Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus, are of major concerns due to increased incidence of water- and seafood-related outbreaks and illness worldwide. Current methods are lengthy and require biochemical and molecular confirmation. A novel label-free forward light-scattering sensor was developed to detect and identify colonies of these three pathogens in real time in the presence of other vibrios in food or water samples. Vibrio colonies grown on agar plates were illuminated by a 635 nm laser beam and scatter-image signatures were acquired using a CCD (charge-coupled device) camera in an automated BARDOT (BActerial Rapid Detection using Optical light-scattering Technology) system. Although a limited number of Vibrio species was tested, each produced a unique light-scattering signature that is consistent from colony to colony. Subsequently a pattern recognition system analysing the collected light-scatter information provided classification in 1-2 min with an accuracy of 99%. The light-scattering signatures were unaffected by subjecting the bacteria to physiological stressors: osmotic imbalance, acid, heat and recovery from a viable but non-culturable state. Furthermore, employing a standard sample enrichment in alkaline peptone water for 6 h followed by plating on selective thiosulphate citrate bile salts sucrose agar at 30°C for ∼ 12 h, the light-scattering sensor successfully detected V. cholerae, V. parahaemolyticus and V. vulnificus present in oyster or water samples in 18 h even in the presence of other vibrios or other bacteria, indicating the suitability of the sensor as a powerful screening tool for pathogens on agar plates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.