ArticleMicrocirculation (New York, N.Y. : 1994)2012
Low proliferative potential and impaired angiogenesis of cultured rat kidney endothelial cells.
Article in Microcirculation (New York, N.Y. : 1994), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 19 citations in OpenAlex.
- Alterations to peritubular capillary structure in a rat model of kidney interstitial fibrosis: Implications for oxygen diffusion.Anatomical record (Hoboken, N.J. : 2007) · 2025Article
- Anemia and Hypoxia Impact on Chronic Kidney Disease Onset and Progression: Review and Updates.Cureus · 2023Review
- Cysteine as a Multifaceted Player in Kidney, theMolecules (Basel, Switzerland) · 2022Review
- Peritubular Capillary Rarefaction: An Underappreciated Regulator of CKD Progression.International journal of molecular sciences · 2020Review
- Rat Mesenchymal Stromal Cell Sheets Suppress Renal Fibrosis via Microvascular Protection.Stem cells translational medicine · 2019Article
- Endothelial colony-forming cells and pro-angiogenic cells: clarifying definitions and their potential role in mitigating acute kidney injury.Acta physiologica (Oxford, England) · 2018Review
- Understanding and managing atherosclerotic renovascular disease: still a work in progress.F1000Research · 2018Review
- Endothelial colony-forming cells ameliorate endothelial dysfunction via secreted factors following ischemia-reperfusion injury.American journal of physiology. Renal physiology · 2017Article
- Small Vessels, Big Role: Renal Microcirculation and Progression of Renal Injury.Hypertension (Dallas, Tex. : 1979) · 2017Review
- Renal endothelial injury and microvascular dysfunction in acute kidney injury.Seminars in nephrology · 2015Review
- Circulating and tissue resident endothelial progenitor cells.Journal of cellular physiology · 2014Review
- Renal endothelial dysfunction in acute kidney ischemia reperfusion injury.Cardiovascular & hematological disorders drug targets · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
objectiveCKD is histologically characterized by interstitial fibrosis, which may be driven by peritubular capillary dropout and hypoxia. Surprisingly, peritubular capillaries have little repair capacity. We sought to establish long-term cultures of rat kidney endothelial cells to investigate their growth regulatory properties.
methodsAKEC or YKEC were isolated using CD31-based isolation techniques and sustained in long-term cultures.
resultsAlthough YKEC grew slightly better than AKEC, both performed poorly compared with endothelial cells of the rat adult PMVEC, PAEC, or HUVEC cells. PMVEC and PAEC contained a large percentage of cells with high colony-forming potential. In contrast, KECs were incapable of forming large colonies and most remained as single nondividing cells. KEC expressed high levels of mRNA for VEGF receptors, but were surprisingly insensitive to VEGF stimulation. KEC did not form branching structures on Matrigel when cultured alone, but in mixed cultures, KEC incorporated into branching structures with PMVEC.
conclusionsThese data suggest that the intrinsic growth of rat kidney endothelial cells is limited by unknown mechanisms. The low growth rate may be related to the minimal intrinsic regenerative capacity of renal capillaries.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.