ArticleJournal of biomedical optics2012
In vivo fluorescence lifetime detection of an activatable probe in infarcted myocardium.
Article in Journal of biomedical optics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- High-speed wide-field fluorescence lifetime imaging for intraoperative tumor visualization and in vivo multiplexing.Biomedical optics express · 2025Article
- Article
- Fluorescence lifetime of injected indocyanine green as a universal marker of solid tumours in patients.Nature biomedical engineering · 2023Article
- First Clinical Results of Fluorescence Lifetime-enhanced Tumor Imaging Using Receptor-targeted Fluorescent Probes.Clinical cancer research : an official journal of the American Association for Cancer Research · 2022Article
- Near-infrared probes for luminescence lifetime imaging.Nanotheranostics · 2022Review
- Molecular probes for selective detection of cysteine cathepsins.Organic & biomolecular chemistry · 2021Review
- The Resolution Matrix in Tomographic Multiplexing: Optimization of Inter-Parameter Cross-Talk, Relative Quantitation, and Localization.IEEE transactions on bio-medical engineering · 2019Article
- Fluorescence lifetime-based contrast enhancement of indocyanine green-labeled tumors.Journal of biomedical optics · 2017Article
- Comparison of tomographic fluorescence spectral and lifetime multiplexing.Optics letters · 2016Article
- Substrate-based near-infrared imaging sensors enable fluorescence lifetime contrast via built-in dynamic fluorescence quenching elements.ACS sensors · 2016Article
- Comparison of Traditional and Integrated Digital Anesthetic Vaporizers.Journal of the American Association for Laboratory Animal Science : JAALAS · 2015Article
- Enzymatically activated near infrared nanoprobes based on amphiphilic block copolymers for optical detection of cancer.Lasers in surgery and medicine · 2015Article
- Molecular probes for fluorescence lifetime imaging.Bioconjugate chemistry · 2015Review
- MMP-2/9-Specific Activatable Lifetime Imaging Agent.Sensors (Basel, Switzerland) · 2015Article
- In vivo tomographic imaging of deep-seated cancer using fluorescence lifetime contrast.Cancer research · 2015Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
Abstract
Activatable fluorescent molecular probes are predominantly nonfluorescent in their inactivated state due to intramolecular quenching, but increase fluorescence yield significantly after enzyme-mediated hydrolysis of peptides. Continuous wave in vivo detection of these protease-activatable fluorophores in the heart, however, is limited by the inability to differentiate between activated and nonactivated fractions of the probe and is frequently complicated by large background signal from probe accumulation in the liver. Using a cathepsin-activatable near-infrared probe (PGC-800), we demonstrate here that fluorescence lifetime (FL) significantly increases in infarcted murine myocardial tissue (0.67 ns) when compared with healthy myocardium (0.59 ns) after 24 h. Furthermore, we show that lifetime contrast can be used to distinguish in vivo cardiac fluorescence from background nonspecific liver signal. The results of this study show that lifetime contrast is a helpful addition to preclinical imaging of activatable fluorophores in the myocardium by reporting molecular activity in vivo due to changes in intramolecular quenching. This characterization of FL from activatable molecular probes will be helpful for advancing in vivo imaging of enzyme activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.