ArticleJournal of tissue engineering and regenerative medicine2012
Comparison of several attachment methods for human iPS, embryonic and adipose-derived stem cells for tissue engineering.
Article in Journal of tissue engineering and regenerative medicine, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed, 85 citations in OpenAlex.
- A human induced pluripotent stem cell model for the holistic study of epithelial-to-mesenchymal transitions.Nature methods · 2026Article
- Poking Pluripotency: Nanoinjection Into Human iPSCs.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Animal-free alternatives for Matrigel in human iPSC-derived blood vessel organoid culture.Scientific reports · 2025Article
- Biocompatible EDOT-Pyrrole Conjugated Conductive Polymer Coating for Augmenting Cell Attachment, Activity, and Differentiation.ACS applied bio materials · 2025Article
- Using Cell and Organ Culture Models to Analyze Responses of Bone Cells to Mechanical Stimulation.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Review
- Control of the growth and development of murine preantral follicles in a biomimetic ovary using a decellularized porcine scaffold.Materials today. Bio · 2023Article
- Vat photopolymerization 3D printed microfluidic devices for organ-on-a-chip applications.Lab on a chip · 2023Review
- LNX2 involves in the role of ghrelin to promote the neuronal differentiation of adipose tissue-derived mesenchymal stem cells.Journal of bioenergetics and biomembranes · 2023Article
- Three-dimensional human bile duct formation from chemically induced human liver progenitor cells.Frontiers in bioengineering and biotechnology · 2023Article
- Surface coating materials regulates the attachment and differentiation of mouse embryonic stem cell derived embryoid bodies into mesoderm at culture conditions.Cytotechnology · 2022Article
- Advancements in Disease Modeling and Drug Discovery Using iPSC-Derived Hepatocyte-like Cells.Genes · 2022Review
- Improving Cell Recovery: Freezing and Thawing Optimization of Induced Pluripotent Stem Cells.Cells · 2022Review
- Reduced growth rate of aged muscle stem cells is associated with impaired mechanosensitivity.Aging · 2022Article
- Rho-Associated Protein Kinase Inhibitor and Hypoxia Synergistically Enhance the Self-Renewal, Survival Rate, and Proliferation of Human Stem Cells.Stem cells and cloning : advances and applications · 2022Article
- Use of standard U-bottom and V-bottom well plates to generate neuroepithelial embryoid bodies.PloS one · 2022Article
- Wafer-Scale Patterning of Protein Templates for Hydrogel Fabrication.Micromachines · 2021Article
- Miniaturized droplet microarray platform enables maintenance of human induced pluripotent stem cell pluripotency.Materials today. Bio · 2021Article
- Mesenchymal Stem Cell and MicroRNA Therapy of Musculoskeletal Diseases.International journal of stem cells · 2021Review
- Caveolin-1 downregulation promotes the dopaminergic neuron-like differentiation of human adipose-derived mesenchymal stem cells.Neural regeneration research · 2021Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
As actual stem cell application quickly approaches tissue engineering and regenerative medicine, aspects such as cell attachment to scaffolds and biomaterials become important and are often overlooked. Here, we compare the effects of several attachment proteins on the adhesion, proliferation and stem cell identity of three promising human stem cell types: human adipose-derived stem cells (hASCs), human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). Traditional tissue culture polystyrene plates (TCPS), Matrigel (Mat), laminin (Lam), fibronectin (FN) and poly-L-lysine (PLL) were investigated as attachment protein surfaces. For hASCs typically cultured on TCPS, laminin resulted in the greatest cell attachment and proliferation with largest cell areas, indicating favourability by cell spreading. However, mesenchymal stem cell markers indicative of hASCs were slightly more expressed on surfaces with lowest cell attachment, corresponding to increased cell roundness, a newly observed attribute in hASCs possibly indicating a more stem cell-like character. hESCs preferred Matrigel as a feeder-free culture surface. Interestingly, hiPSCs favoured laminin over Matrigel for colony expansion, shown by larger cell colony area and perimeter lengths, although cell numbers and stem cell marker expression level remained highest on Matrigel. These data provide a practical reference guide for selecting a suitable attachment method for using human induced pluripotent, embryonic or adipose stem cells in tissue engineering and regenerative medicine applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.