ArticleApplied and environmental microbiology2012
One-step sequence- and ligation-independent cloning as a rapid and versatile cloning method for functional genomics studies.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 241 papers.
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Who cites it
241 citing papers in PubMed.
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- "Molecular insights into the bactericidal toxin Tle1 of Pseudomonas aeruginosa: Interaction with VgrG, its adaptor, and its immunity protein".The Journal of biological chemistry · 2026Article
- Unveiling a missing component of the atypical type IV secretion system required for natural transformation ofbioRxiv : the preprint server for biology · 2026Article
- Unexpected Dual Function of Plant YUCCA Enzymes Links Chlorophyll Catabolism to Auxin Homeostasis.Angewandte Chemie (International ed. in English) · 2026Article
- Pseudopaline-mediated zinc uptake byInfection and immunity · 2026Article
- Human FASTK preferentially binds single-stranded and G-rich RNA.The FEBS journal · 2026Article
- Association-induced folding governs surrogate light chain and pre-B cell receptor core assembly.Nature communications · 2026Article
- Activation of the pituitary adenylate cyclase-activating polypeptide type I receptor promotes neuroblastoma proliferation and migration through distinct G protein pathways.Cell communication and signaling : CCS · 2026Article
- Seamless, modular binary vectors for plant cell and molecular biology.Plant biotechnology journal · 2026Article
- The CRISPR/Cas-associated scaRNA modulatesmicroLife · 2026Article
- Phenotypic and transcriptomic characterization of OsSWEET14-edited rice (cv. Samkwang) with enhanced bacterial blight resistance.BMC plant biology · 2025Article
- Homology-mediated transformation of frog-killing fungusProceedings of the National Academy of Sciences of the United States of America · 2025Article
181 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed one-step sequence- and ligation-independent cloning (SLIC) as a simple, cost-effective, time-saving, and versatile cloning method. Highly efficient and directional cloning can be achieved by direct bacterial transformation 2.5 min after mixing any linearized vector, an insert(s) prepared by PCR, and T4 DNA polymerase in a tube at room temperature.
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Registered trials
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