ArticleApplied and environmental microbiology2012
Development of an efficient in vivo system (Pjunc-TpaseIS1223) for random transposon mutagenesis of Lactobacillus casei.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed.
- Illuminating the oral microbiome and its host interactions: tools and approaches for molecular microbiology studies.FEMS microbiology reviews · 2023Review
- Resistance to host antimicrobial peptides mediates resilience of gut commensals during infection and aging inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Recent advances in genetic tools for engineering probiotic lactic acid bacteria.Bioscience reports · 2023Article
- Novel shuttle vector pGMβ1 for conjugative chromosomal manipulation ofBioscience of microbiota, food and health · 2022Article
- Study of theFrontiers in microbiology · 2020Article
- Article
- Targeted and Repetitive Chromosomal Integration Enables High-Level Heterologous Gene Expression inApplied and environmental microbiology · 2019Article
- A Transposon Mutagenesis System for Bifidobacterium longum subsp. longum Based on an ISApplied and environmental microbiology · 2018Article
- New Genes Involved in Mild Stress Response Identified by Transposon Mutagenesis inFrontiers in microbiology · 2018Article
- D-Alanylation of teichoic acids contributes to Lactobacillus plantarum-mediated Drosophila growth during chronic undernutrition.Nature microbiology · 2017Article
- Functional genomics of Lactobacillus casei establishment in the gut.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Identification of a proton-chloride antiporter (EriC) by Himar1 transposon mutagenesis in Lactobacillus reuteri and its role in histamine production.Antonie van Leeuwenhoek · 2014Article
- Identification of critical genes for growth in olive brine by transposon mutagenesis of Lactobacillus pentosus C11.Applied and environmental microbiology · 2013Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The random transposon mutagenesis system P(junc)-TpaseIS(1223) is composed of plasmids pVI129, expressing IS1223 transposase, and pVI110, a suicide transposon plasmid carrying the P(junc) sequence, the substrate of the IS1223 transposase. This system is particularly efficient in Lactobacillus casei, as more than 10,000 stable, random mutants were routinely obtained via electroporation.
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Registered trials
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