ArticleJournal of bacteriology2012
Degradation of 2,3-dihydroxybenzoate by a novel meta-cleavage pathway.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 27 citations in OpenAlex.
- Genomic exploration of Serratia rubidaea MJ24 uncovers diverse biosynthetic capacity and agricultural relevance.Functional & integrative genomics · 2026Article
- A novel phthalic acid degradation pathway in Klebsiella variicola SY1 and engineering the strain for degradation of multiple phthalate esters.World journal of microbiology & biotechnology · 2026Article
- Screening and dynamic change study of microbial and metabolite markers for calf diarrhea based on multi-omics and machine learning.mSystems · 2026Article
- Physicochemistry and comparative metagenomics of a tropical estuary persistently inundated with anthropogenic pollutants.Folia microbiologica · 2025Article
- Biotechnological Production, Isolation and Characterisation of (2R,3S)-2,3-Dihydroxy-2,3-Dihydrobenzoate.Microbial biotechnology · 2025Article
- The enterobactin biosynthetic intermediate 2,3-dihydroxybenzoic acid is a competitive inhibitor of the Escherichia coli isochorismatase EntB.Protein science : a publication of the Protein Society · 2025Article
- Metabolic engineering ofProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Mechanisms and high-value applications of phthalate isomers degradation pathways in bacteria.World journal of microbiology & biotechnology · 2024Review
- Aerobic phenol degradation using native bacterial consortium via ortho-and meta-cleavage pathways.Frontiers in microbiology · 2024Article
- Microwell Fluoride Screen for Chemical, Enzymatic, and Cellular Reactions Reveals Latent Microbial Defluorination Capacity for -CFApplied and environmental microbiology · 2022Article
- Bioprocess performance, transformation pathway, and bacterial community dynamics in an immobilized cell bioreactor treating fludioxonil-contaminated wastewater under microaerophilic conditions.Environmental science and pollution research international · 2022Article
- A metabolomics approach to evaluate the effect of lyophilization versus oven drying on the chemical composition of plant extracts.Scientific reports · 2021Article
- Article
- Oxidative opening of the aromatic ring: Tracing the natural history of a large superfamily of dioxygenase domains and their relatives.The Journal of biological chemistry · 2019Article
- 2,3-Dihydroxybenzoate meta-Cleavage Pathway is Involved in o-Phthalate Utilization in Pseudomonas sp. strain PTH10.Scientific reports · 2019Article
- Prediction of Host-Specific Genes by Pan-Genome Analyses of the KoreanFrontiers in microbiology · 2019Article
- Metabolic functions of Pseudomonas fluorescens strains from Populus deltoides depend on rhizosphere or endosphere isolation compartment.Frontiers in microbiology · 2015Article
- Biodegradation of polycyclic aromatic hydrocarbons by Novosphingobium pentaromativorans US6-1.PloS one · 2014Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
2,3-Dihydroxybenzoate is the precursor in the biosynthesis of several siderophores and an important plant secondary metabolite that, in bacteria, can be degraded via meta-cleavage of the aromatic ring. The dhb cluster of Pseudomonas reinekei MT1 encodes a chimeric meta-cleavage pathway involved in the catabolism of 2,3-dihydroxybenzoate. While the first two enzymes, DhbA and DhbB, are phylogenetically related to those involved in 2,3-dihydroxy-p-cumate degradation, the subsequent steps are catalyzed by enzymes related to those involved in catechol degradation (DhbCDEFGH). Characterization of kinetic properties of DhbA extradiol dioxygenase identified 2,3-dihydroxybenzoate as the preferred substrate. Deletion of the encoding gene impedes growth of P. reinekei MT1 on 2,3-dihydroxybenzoate. DhbA catalyzes 3,4-dioxygenation with 2-hydroxy-3-carboxymuconate as the product, which is then decarboxylated by DhbB to 2-hydroxymuconic semialdehyde. This compound is then subject to dehydrogenation and further degraded to citrate cycle intermediates. Transcriptional analysis revealed genes of the dhB gene cluster to be highly expressed during growth with 2,3-dihydroxybenzoate, whereas a downstream-localized gene encoding 2-hydroxymuconic semialdehyde hydrolase, dispensable for 2,3-dihydroxybenzoate metabolism but crucial for 2,3-dihydroxy-p-cumate degradation, was only marginally expressed. This is the first report describing a gene cluster encoding enzymes for the degradation of 2,3-dihydroxybenzoate.
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