ArticleJournal of bacteriology2012
Extracellular identification of a processed type II ComR/ComS pheromone of Streptococcus mutans.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 72 citations in OpenAlex.
- Environmental Control of Queuosine Levels in Streptococcus mutans tRNAs.Molecular microbiology · 2025Article
- Noncanonical Streptococcus sanguinis ComCDE circuitry integrates environmental cues in transformation outcome decision.Cell chemical biology · 2024Article
- In Vitro Impact of Fluconazole on Oral Microbial Communities, Bacterial Growth, and Biofilm Formation.Antibiotics (Basel, Switzerland) · 2023Article
- Mucin O-glycans suppress quorum-sensing pathways and genetic transformation in Streptococcus mutans.Nature microbiology · 2021Article
- Direct interactions with commensal streptococci modify intercellular communication behaviors of Streptococcus mutans.The ISME journal · 2021Article
- Structure Activity Relationship Study of the XIP Quorum Sensing Pheromone inACS chemical biology · 2020Article
- Article
- Molecular dissection of pheromone selectivity in the competence signaling system ComRS of streptococci.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Singularities of Pyogenic Streptococcal Biofilms - From Formation to Health Implication.Frontiers in microbiology · 2020Review
- The Biology ofMicrobiology spectrum · 2019Article
- Expanding the Vocabulary of Peptide Signals inFrontiers in cellular and infection microbiology · 2019Review
- Conserved Pheromone Production, Response and Degradation byFrontiers in microbiology · 2019Article
- The conserved mosaic prophage protein paratox inhibits the natural competence regulator ComR in Streptococcus.Scientific reports · 2018Article
- Intracellular Signaling by themSphere · 2018Article
- Competence inhibition by the XrpA peptide encoded within the comX gene of Streptococcus mutans.Molecular microbiology · 2018Article
- Getting to Know "The Known Unknowns": Heterogeneity in the Oral Microbiome.Advances in dental research · 2018Review
- Hidden Gems in the Transcriptome Maps of Competent Streptococci.Frontiers in molecular biosciences · 2018Article
- Curcumin as a Promising Antibacterial Agent: Effects on Metabolism and Biofilm Formation inBioMed research international · 2018Article
- Intercellular Communication via theJournal of bacteriology · 2017Article
- A positive feedback loop mediated by Sigma X enhances expression of the streptococcal regulator ComR.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The competence-stimulating peptide (CSP) and the sigX-inducing peptide (XIP) are known to induce Streptococcus mutans competence for genetic transformation. For both pheromones, direct identification of the native peptides has not been accomplished. The fact that extracellular XIP activity was recently observed in a chemically defined medium devoid of peptides, as mentioned in an accompanying paper (K. Desai, L. Mashburn-Warren, M. J. Federle, and D. A. Morrison, J. Bacteriol. 194:3774-3780, 2012), provided ideal conditions for native XIP identification. To search for the XIP identity, culture supernatants were filtered to select for peptides of less than 3 kDa, followed by C(18) extraction. One peptide, not detected in the supernatant of a comS deletion mutant, was identified by tandem mass spectrometry (MS/MS) fragmentation as identical to the ComS C-terminal sequence GLDWWSL. ComS processing did not require Eep, a peptidase involved in processing or import of bacterial small hydrophobic peptides, since eep deletion had no inhibitory effect on XIP production or on synthetic XIP response. We investigated whether extracellular CSP was also produced. A reporter assay for CSP activity detection, as well as MS analysis of supernatants, revealed that CSP was not present at detectable levels. In addition, a mutant with deletion of the CSP-encoding gene comC produced endogenous XIP levels similar to those of a nondeletion mutant. The results indicate that XIP pheromone production is a natural phenomenon that may occur in the absence of natural CSP pheromone activity and that the heptapeptide GLDWWSL is an extracellular processed form of ComS, possibly the active XIP pheromone. This is the first report of direct identification of a ComR/ComS pheromone.
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