ArticleIntegrative biology : quantitative biosciences from nano to macro2012
Systematic analysis of embryonic stem cell differentiation in hydrodynamic environments with controlled embryoid body size.
Article in Integrative biology : quantitative biosciences from nano to macro, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 44 citations in OpenAlex.
- Microfluidic perfusion modulates growth and motor neuron differentiation of stem cell aggregates.The Analyst · 2020Article
- Hydrodynamic shear stress promotes epithelial-mesenchymal transition by downregulating ERK and GSK3β activities.Breast cancer research : BCR · 2019Article
- Towards Multi-Organoid Systems for Drug Screening Applications.Bioengineering (Basel, Switzerland) · 2018Review
- Statistical analysis of multi-dimensional, temporal gene expression of stem cells to elucidate colony size-dependent neural differentiation.Molecular omics · 2018Article
- A microfluidic trap array for longitudinal monitoring and multi-modal phenotypic analysis of individual stem cell aggregates.Lab on a chip · 2017Article
- The role of adhesion junctions in the biomechanical behaviour and osteogenic differentiation of 3D mesenchymal stem cell spheroids.Journal of biomechanics · 2017Article
- Concise Review: Stem Cell Microenvironment on a Chip: Current Technologies for Tissue Engineering and Stem Cell Biology.Stem cells translational medicine · 2015Review
- Quantitative multivariate analysis of dynamic multicellular morphogenic trajectories.Integrative biology : quantitative biosciences from nano to macro · 2015Article
- Osteogenic embryoid body-derived material induces bone formation in vivo.Scientific reports · 2015Article
- Controlling expansion and cardiomyogenic differentiation of human pluripotent stem cells in scalable suspension culture.Stem cell reports · 2014Article
- Differential expression of extracellular matrix and growth factors by embryoid bodies in hydrodynamic and static cultures.Tissue engineering. Part C, Methods · 2014Article
- Organ-on-a-chip platforms for studying drug delivery systems.Journal of controlled release : official journal of the Controlled Release Society · 2014Review
- Mesenchymal morphogenesis of embryonic stem cells dynamically modulates the biophysical microtissue niche.Scientific reports · 2014Article
- Alginate encapsulation parameters influence the differentiation of microencapsulated embryonic stem cell aggregates.Biotechnology and bioengineering · 2014Article
- Fluid shear stress pre-conditioning promotes endothelial morphogenesis of embryonic stem cells within embryoid bodies.Tissue engineering. Part A · 2014Article
- Engineering three-dimensional stem cell morphogenesis for the development of tissue models and scalable regenerative therapeutics.Annals of biomedical engineering · 2014Review
- Single-cell analysis of embryoid body heterogeneity using microfluidic trapping array.Biomedical microdevices · 2014Article
- Impaired bone formation in Pdia3 deficient mice.PloS one · 2014Article
- Microbead-based biomimetic synthetic neighbors enhance survival and function of rat pancreatic β-cells.Scientific reports · 2013Article
- Temporal modulation of β-catenin signaling by multicellular aggregation kinetics impacts embryonic stem cell cardiomyogenesis.Stem cells and development · 2013Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
The sensitivity of stem cells to environmental perturbations has prompted many studies which aim to characterize the influence of mechanical factors on stem cell morphogenesis and differentiation. Hydrodynamic cultures, often employed for large scale bioprocessing applications, impart complex fluid shear and transport profiles, and influence cell fate as a result of changes in media mixing conditions. However, previous studies of hydrodynamic cultures have been limited in their ability to distinguish confounding factors that may affect differentiation, including modulation of embryoid body size in response to changes in the hydrodynamic environment. In this study, we demonstrate the ability to control and maintain embryoid body (EB) size using a combination of forced aggregation formation and rotary orbital suspension culture, in order to assess the impact of hydrodynamic cultures on ESC differentiation, independent of EB size. Size-controlled EBs maintained at different rotary orbital speeds exhibited similar morphological features and gene expression profiles, consistent with ESC differentiation. The similar differentiation of ESCs across a range of hydrodynamic conditions suggests that controlling EB formation and resultant size may be important for scalable bioprocessing applications, in order to standardize EB morphogenesis. However, perturbations in the hydrodynamic environment also led to subtle changes in differentiation toward certain lineages, including temporal modulation of gene expression, as well changes in the relative efficiencies of differentiated phenotypes, thereby highlighting important tissue engineering principles that should be considered for implementation in bioreactor design, as well as for directed ESC differentiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.