ArticleBiochimica et biophysica acta2012
A role for the vesicle-associated tubulin binding protein ARL6 (BBS3) in flagellum extension in Trypanosoma brucei.
Article in Biochimica et biophysica acta, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 24 citations in OpenAlex.
- Article
- Identification and bioinformatics analysis of cilia-associated gene families inFrontiers in microbiology · 2025Article
- A Not-So-Ancient Grease History: Click Chemistry and Protein Lipid Modifications.Chemical reviews · 2021Review
- Article
- Defeating the trypanosomatid trio: proteomics of the protozoan parasites causing neglected tropical diseases.RSC medicinal chemistry · 2020Review
- APOL1 C-Terminal Variants May Trigger Kidney Disease through Interference with APOL3 Control of Actomyosin.Cell reports · 2020Article
- Genetic validation of Leishmania genes essential for amastigote survival in vivo using N-myristoyltransferase as a model.Parasites & vectors · 2020Article
- The Joubert syndrome protein ARL13B binds tubulin to maintain uniform distribution of proteins along the ciliary membrane.Journal of cell science · 2018Article
- Guanine Nucleotide Exchange Assay Using Fluorescent MANT-GDP.Bio-protocol · 2018Article
- Global Profiling and Inhibition of Protein Lipidation in Vector and Host Stages of the Sleeping Sickness ParasiteACS infectious diseases · 2016Article
- Loss of the BBSome perturbs endocytic trafficking and disrupts virulence of Trypanosoma brucei.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Ca2+ Regulation of Trypanosoma brucei Phosphoinositide Phospholipase C.Eukaryotic cell · 2015Article
- Global analysis of protein N-myristoylation and exploration of N-myristoyltransferase as a drug target in the neglected human pathogen Leishmania donovani.Chemistry & biology · 2015Article
- Structure-based design of potent and selective Leishmania N-myristoyltransferase inhibitors.Journal of medicinal chemistry · 2014Article
- Motility and more: the flagellum of Trypanosoma brucei.Nature reviews. Microbiology · 2014Review
- The Leishmania major BBSome subunit BBS1 is essential for parasite virulence in the mammalian host.Molecular microbiology · 2013Article
- Crystal structure of the small GTPase Arl6/BBS3 from Trypanosoma brucei.Protein science : a publication of the Protein Society · 2013Article
- Article
- Imaging African trypanosomes.Parasite immunologyReview
- High yield production of myristoylated Arf6 small GTPase by recombinant N-myristoyl transferase.Small GTPasesArticle
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Authors and funding
8 authors at 3 institutions in 1 country.
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Abstract
The small GTPase Arl6 is implicated in the ciliopathic human genetic disorder Bardet-Biedl syndrome, acting at primary cilia in recruitment of the octomeric BBSome complex, which is required for specific trafficking events to and from the cilium in eukaryotes. Here we describe functional characterisation of Arl6 in the flagellated model eukaryote Trypanosoma brucei, which requires motility for viability. Unlike human Arl6 which has a ciliary localisation, TbARL6 is associated with electron-dense vesicles throughout the cell body following co-translational modification by N-myristoylation. Similar to the related protein ARL-3A in T. brucei, modulation of expression of ARL6 by RNA interference does not prevent motility but causes a significant reduction in flagellum length. Tubulin is identified as an ARL6 interacting partner, suggesting that ARL6 may act as an anchor between vesicles and cytoplasmic microtubules. We provide evidence that the interaction between ARL6 and the BBSome is conserved in unicellular eukaryotes. Overexpression of BBS1 leads to translocation of endogenous ARL6 to the site of exogenous BBS1 at the flagellar pocket. Furthermore, a combination of BBS1 overexpression and ARL6 RNAi has a synergistic inhibitory effect on cell growth. Our findings indicate that ARL6 in trypanosomes contributes to flagellum biogenesis, most likely through an interaction with the BBSome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.