ArticleOphthalmology2012
Cellular changes of the corneal epithelium and stroma in herpes simplex keratitis: an in vivo confocal microscopy study.
Article in Ophthalmology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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Who cites it
28 citing papers in PubMed, 67 citations in OpenAlex.
- Current microfluidic platforms for reverse engineering of cornea.Materials today. Bio · 2023Review
- Viral Infection and Antiviral Treatments in Ocular Pathologies.Microorganisms · 2022Review
- Immune responses to injury and their links to eye disease.Translational research : the journal of laboratory and clinical medicine · 2021Review
- Bilateral Limbal Stem Cell Alterations in Patients With Unilateral Herpes Simplex Keratitis and Herpes Zoster Ophthalmicus as Shown by In Vivo Confocal Microscopy.Investigative ophthalmology & visual science · 2021Article
- Pseudomonas aeruginosa-induced nociceptor activation increases susceptibility to infection.PLoS pathogens · 2021Article
- Unilateral Corneal Insult Also Alters Sensory Nerve Activity in the Contralateral Eye.Frontiers in medicine · 2021Article
- Deep learning-based automated diagnosis of fungal keratitis withAnnals of translational medicine · 2020Article
- Resolution of herpes simplex virus reactivation in vivo results in neuronal destruction.PLoS pathogens · 2020Article
- NFE2L2 activator RS9 protects against corneal epithelial cell damage in dry eye models.PloS one · 2020Article
- Corneal Sub-Basal Nerve Changes in Patients with Herpetic Keratitis During Acute Phase and after 6 Months.Medicina (Kaunas, Lithuania) · 2019Article
- Corneal esthesiometry and sub-basal nerves morphological changes in herpes simplex virus keratitis/uveitis patients.International journal of ophthalmology · 2019Article
- Corneal nerve regeneration after herpes simplex keratitis: A longitudinal in vivo confocal microscopy study.The ocular surface · 2018Article
- Impact of herpetic stromal immune keratitis in corneal biomechanics and innervation.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2018Observational
- Self-Retained Amniotic Membrane Combined With Antiviral Therapy for Herpetic Epithelial Keratitis.Cornea · 2017Article
- In vivo Confocal Microscopy of Posner-Schlossman Syndrome: Comparison with herpes simplex keratitis, HLA-B27 anterior uveitis and acute attack of primary angle closure.Scientific reports · 2017Article
- Understanding Neuropathic Corneal Pain--Gaps and Current Therapeutic Approaches.Seminars in ophthalmology · 2016Review
- Challenges of corneal infections.Expert review of ophthalmology · 2016Article
- Comparison of Standard Versus Wide-Field Composite Images of the Corneal Subbasal Layer by In Vivo Confocal Microscopy.Investigative ophthalmology & visual science · 2015Article
- In vivo detection of clinically non-apparent ocular surface inflammation in patients with meibomian gland dysfunction-associated refractory dry eye symptoms: a pilot study.Eye (London, England) · 2015Observational
- In Vivo Confocal Microscopy Demonstrates Bilateral Loss of Endothelial Cells in Unilateral Herpes Simplex Keratitis.Investigative ophthalmology & visual science · 2015Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
purposeTo analyze the morphologic features of corneal epithelial cells and keratocytes by in vivo confocal microscopy in patients with herpes simplex keratitis (HSK) as associated with corneal innervation.
designProspective, cross-sectional, controlled, single-center study.
participantsThirty-one eyes with the diagnosis HSK and their contralateral clinically unaffected eyes were studied and compared with normal controls (n = 15).
methodsIn vivo confocal microscopy (Confoscan 4; Nidek Technologies, Gamagori, Japan) and corneal esthesiometry (Cochet-Bonnet; Luneau Ophthalmologie, Chartres, France) of the central cornea were performed bilaterally in all patients and controls. Patients were grouped into normal (>5.5 cm), mild (>2.5-5.5 cm), and severe (<2.5 cm) loss of sensation.
main outcome measuresChanges in morphologic features and density of the superficial and basal epithelial cells, as well as stromal keratocytes, were assessed by 2 masked observers. Changes were correlated to corneal sensation, number of nerves, and total length of nerves.
resultsThere was a significant and gradual decrease in the density of superficial epithelial cells in HSK eyes, with 852.50 ± 24.4 cells/mm(2) in eyes with severe sensation loss and 2435.23 ± 224.3 cells/mm(2) in control eyes (P = 0.008). Superficial epithelial cell size was 2.5-fold larger in HSK eyes (835.3 μm(2)) compared with contralateral or normal eyes (407.4 μm(2); P = 0.003). A significant number of hyperreflective desquamating superficial epithelial cells were present in HSK eyes with normal (6.4%), mild (29.1%), and severe (52.2%) loss of sensation, but were absent in controls. The density of basal epithelial cells, anterior keratocytes, and posterior keratocytes did not show statistical significance between patients and controls. Changes in superficial epithelial cell density and morphologic features correlated strongly with total nerve length, number, and corneal sensation. Scans of contralateral eyes did not show any significant epithelial or stromal changes compared with controls.
conclusionsIn vivo confocal microscopy reveals profound HSK-induced changes in the superficial epithelium, as demonstrated by increase in cell size, decrease in cell density, and squamous metaplasia. This study demonstrated that these changes correlate strongly with changes in corneal innervation.
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