ArticleTissue engineering. Part A2012
Engineering muscle tissues on microstructured polyelectrolyte multilayer films.
Article in Tissue engineering. Part A, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 40 citations in OpenAlex.
- Ultrafast Micrometric Laser Structuring of Polymer Surfaces for Highly Organized Skeletal Muscle Tissue Modeling.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Cytotoxic and Mechanical Properties of Resins 3D Printed with Low-Cost Hardware for C2C12 Biohybrid Actuators.Scientific data · 2025Article
- Micropatterned Styrene-Butadiene-Styrene Thin Films Doped with Barium Titanate Nanoparticles: Effects on Myoblast Differentiation.ACS biomaterials science & engineering · 2025Article
- Developing Porous Fibrin Scaffolds with Tunable Anisotropic Features to Direct Myoblast Orientation.Tissue engineering. Part C, Methods · 2024Article
- Next Stage Approach to Tissue Engineering Skeletal Muscle.Bioengineering (Basel, Switzerland) · 2020Review
- Skeletal Muscle Tissue Engineering: Biomaterials-Based Strategies for the Treatment of Volumetric Muscle Loss.Bioengineering (Basel, Switzerland) · 2020Review
- New volumetric CNT-doped gelatin-cellulose scaffolds for skeletal muscle tissue engineering.Nanoscale advances · 2020Article
- Etching anisotropic surface topography onto fibrin microthread scaffolds for guiding myoblast alignment.Journal of biomedical materials research. Part B, Applied biomaterials · 2020Article
- The Importance of Biophysical and Biochemical Stimuli in Dynamic Skeletal Muscle Models.Frontiers in physiology · 2018Review
- Quiescence of human muscle stem cells is favored by culture on natural biopolymeric films.Stem cell research & therapy · 2017Article
- Achieving Acetylcholine Receptor Clustering in Tissue-Engineered Skeletal Muscle ConstructsFrontiers in pharmacology · 2016Article
- Spatio-Temporal Control of LbL Films for Biomedical Applications: From 2D to 3D.Advanced healthcare materials · 2015Review
- Engineering muscle constructs for the creation of functional engineered musculoskeletal tissue.Regenerative medicine · 2014Review
- Skeletal muscle tissue engineering: strategies for volumetric constructs.Frontiers in physiology · 2014Review
- Release retardation of model protein on polyelectrolyte-coated PLGA nano- and microparticles.PloS one · 2014Article
- Rigidity-patterned polyelectrolyte films to control myoblast cell adhesion and spatial organization.Advanced functional materials · 2013Article
- Effect of RGD functionalization and stiffness modulation of polyelectrolyte multilayer films on muscle cell differentiation.Acta biomaterialia · 2013Article
- Gradients of physical and biochemical cues on polyelectrolyte multilayer films generated via microfluidics.Lab on a chip · 2013Article
- Boron nitride nanotube-mediated stimulation of cell co-culture on micro-engineered hydrogels.PloS one · 2013Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The use of surface coating on biomaterials can render the original substratum with new functionalities that can improve the chemical, physical, and mechanical properties as well as enhance cellular cues such as attachment, proliferation, and differentiation. In this work, we combined biocompatible polydimethylsiloxane (PDMS) with a biomimetic polyelectrolyte multilayer (PEM) film made of poly(L-lysine) and hyaluronic acid (PLL/HA) for skeletal muscle tissue engineering. By microstructuring PDMS in grooves of a different width (5, 10, 30, and 100 μm) and by modulating the stiffness of the (PLL/HA) films, we guided skeletal muscle cell differentiation into myotubes. We found optimal conditions for both the formation of parallel-oriented myotubes and their maturation. Significantly, the myoblasts were collectively prealigned to the grooves before their differentiation. Before fusion, the highest aspect ratio and orientation of nuclei were observed for the 5 and 10 μm wide micropatterns. The formation of myotubes was observed regardless of the size of the micropatterns, and we found that their typical width was 10-12 μm. Their maturation was characterized by the immunolabeling of type II isomyosin. The amount of myosin striation was not affected by the topography, except for the 5 μm wide micropatterns. We highlighted the spatial constraints that led to an important nuclei deformation and further impairment of maturation within the 5 μm grooves. Altogether, our results show that the PEM film combined with PDMS is a powerful tool that is used for skeletal muscle engineering. This work opens perspectives for the development of skeletal muscle tissue in contact with films containing bioactive peptides or growth factors as well as for the study of pathogenic myotubes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.