ArticlePloS one2012
A multi-platform flow device for microbial (co-) cultivation and microscopic analysis.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- Strategies for Actinobacteria Isolation, Cultivation, and Metabolite Production that Are Biologically Important.ACS omega · 2025Review
- Float-Cast Microsieves with Elliptical Pores.Langmuir : the ACS journal of surfaces and colloids · 2024Article
- A Two-Compartment Fermentation System to Quantify Strain-Specific Interactions in Microbial Co-Cultures.Bioengineering (Basel, Switzerland) · 2023Article
- Genomic insights into versatile lifestyle of three new bacterial candidate phyla.Science China. Life sciences · 2022Article
- Microbial factories: monitoring vitamin BLab on a chip · 2021Article
- Diversity, metabolism and cultivation of archaea in mangrove ecosystems.Marine life science & technology · 2021Review
- Microfluidic and mathematical modeling of aquatic microbial communities.Analytical and bioanalytical chemistry · 2021Review
- No man's land: Species-specific formation of exclusion zones bordering Actinomyces graevenitzii microcolonies in nanoliter cultures.MicrobiologyOpen · 2021Article
- Diversity and distribution of marine heterotrophic bacteria from a large culture collection.BMC microbiology · 2020Article
- Cross-Flow Filtration ofMicromachines · 2019Article
- Separation and Detection ofBiosensors · 2019Article
- Integration of mesenchymal stem cells into islet cell spheroids improves long-term viability, but not islet function.Islets · 2017Article
- Physically Triggered Morphology Changes in a NovelFrontiers in microbiology · 2017Article
- Decolorization of Selected Synthetic Textile Dyes by Yeasts from Leaves and Fruit Peels.Journal of health & pollution · 2016Article
- Co-culture systems and technologies: taking synthetic biology to the next level.Journal of the Royal Society, Interface · 2014Review
- Design and analysis of a tunable synchronized oscillator.Journal of biological engineering · 2013Article
- The Constructor: a web application optimizing cloning strategies based on modules from the registry of standard biological parts.Journal of biological engineering · 2012Article
- Review
Corrections and comments
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Authors and funding
19 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Novel microbial cultivation platforms are of increasing interest to researchers in academia and industry. The development of materials with specialized chemical and geometric properties has opened up new possibilities in the study of previously unculturable microorganisms and has facilitated the design of elegant, high-throughput experimental set-ups. Within the context of the international Genetically Engineered Machine (iGEM) competition, we set out to design, manufacture, and implement a flow device that can accommodate multiple growth platforms, that is, a silicon nitride based microsieve and a porous aluminium oxide based microdish. It provides control over (co-)culturing conditions similar to a chemostat, while allowing organisms to be observed microscopically. The device was designed to be affordable, reusable, and above all, versatile. To test its functionality and general utility, we performed multiple experiments with Escherichia coli cells harboring synthetic gene circuits and were able to quantitatively study emerging expression dynamics in real-time via fluorescence microscopy. Furthermore, we demonstrated that the device provides a unique environment for the cultivation of nematodes, suggesting that the device could also prove useful in microscopy studies of multicellular microorganisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.