ArticleInternational journal of molecular sciences2012
The establishment of a primary culture system of proximal tubule segments using specific markers from normal mouse kidneys.
Article in International journal of molecular sciences, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 43 citations in OpenAlex.
- ANXA1 improves mitochondrial homeostasis through uncoupling protein 1 in diabetic nephropathy.Journal of advanced research · 2026Article
- Isolation and functional characterization of primary human proximal tubular epithelial cells from brain-dead organ donors.Physiological reports · 2026Article
- hTERT and SV40LgT Renal Cell Lines Adjust Their Transcriptional Responses After Copy Number Changes from the Parent Proximal Tubule Cells.International journal of molecular sciences · 2025Article
- Activation of Purinergic P2Y2 Receptor Protects the Kidney Against Renal Ischemia and Reperfusion Injury in Mice.International journal of molecular sciences · 2024Article
- Enhancing maturity in 3D kidney micro-tissues through clonogenic cell combinations and endothelial integration.Journal of cellular and molecular medicine · 2024Article
- The Rise in Tubular pH during Hypercalciuria Exacerbates Calcium Stone Formation.International journal of molecular sciences · 2024Article
- Determining epigenetic memory in kidney proximal tubule cell derived induced pluripotent stem cells using a quadruple transgenic reprogrammable mouse.Scientific reports · 2022Article
- Inactivation of fatty acid amide hydrolase protects against ischemic reperfusion injury-induced renal fibrogenesis.Biochimica et biophysica acta. Molecular basis of disease · 2022Article
- Hypercalciuria switches CaGenes & diseases · 2022Article
- Flipped Well-Plate Hanging-Drop Technique for Growing Three-Dimensional Tumors.Frontiers in bioengineering and biotechnology · 2022Article
- Non-invasive assessment of exfoliated kidney cells extracted from urine using multispectral autofluorescence features.Scientific reports · 2021Article
- Dapagliflozin Ameliorates Diabetic Kidney DiseaseFrontiers in pharmacology · 2021Article
- Epigenetic Regulation of KL (Klotho) via H3K27me3 (Histone 3 Lysine [K] 27 Trimethylation) in Renal Tubule Cells.Hypertension (Dallas, Tex. : 1979) · 2020Article
- Blockade of sodium-glucose cotransporter 2 suppresses high glucose-induced angiotensinogen augmentation in renal proximal tubular cells.American journal of physiology. Renal physiology · 2020Article
- Evidence for a regulated CaJournal of cell science · 2019Article
- EGF Receptor-Dependent YAP Activation Is Important for Renal Recovery from AKI.Journal of the American Society of Nephrology : JASN · 2018Article
- Isolation, Characterization, And High Throughput Extracellular Flux Analysis of Mouse Primary Renal Tubular Epithelial Cells.Journal of visualized experiments : JoVE · 2018Article
- Osteopontin deficiency ameliorates Alport pathology by preventing tubular metabolic deficits.JCI insight · 2018Article
- Differentiating mouse embryonic stem cells express markers of human endometrium.Reproductive biology and endocrinology : RB&E · 2017Article
- Application of Hanging Drop Technique for Kidney Tissue Culture.Kidney & blood pressure research · 2017Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The proximal tubule contains the highest expression of angiotensinogen mRNA and protein within the kidney and plays a vital role in the renal renin-angiotensin system. To study the regulation of angiotensinogen expression in the kidney in more detail, the proximal tubule needs to be accurately isolated from the rest of the nephron and separated into its three segments. The purpose of this study was to design a novel protocol using specific markers for the separation of proximal tubule cells into the three proximal tubule segments and to determine angiotensinogen expression in each segment. Kidneys were removed from C57BL/6J mice. The proximal tubules were aspirated from region of a Percoll gradient solution of the appropriate density. The proximal tubule was then separated into its three segments using segment-specific membrane proteins, after which each segment was characterized by a different specific marker (sodium-glucose transporter 2 for Segment 1; carbonic anhydrase IV for Segment 2; ecto-adenosine triphosphatase for Segment 3). The isolation of proximal tubules into three segments was successful, and angiotensinogen mRNA in Segment 2 and 3 and angiotensinogen protein in all three segments were confirmed. This protocol will be helpful for future studies of the detailed mechanisms of the intrarenal renin-angiotensin system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.