ArticleInternational journal of molecular sciences2012
Expression of Transient Receptor Potential Vanilloid (TRPV) channels in different passages of articular chondrocytes.
Article in International journal of molecular sciences, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 35 citations in OpenAlex.
- Review
- Tracking chondrocyte-to-fibroblast transformation via changes in cell electrophysiology.Scientific reports · 2025Article
- Role of transient receptor potential (TRP) channels in osteoarthritis: a comprehensive review.Frontiers in pharmacology · 2025Review
- Interplay of Glucose Metabolism and Hippo Pathway in Chondrocytes: Pathophysiology and Therapeutic Targets.Bioengineering (Basel, Switzerland) · 2024Review
- Ion channels in osteoarthritis: emerging roles and potential targets.Nature reviews. Rheumatology · 2024Review
- TRPV Channels in Osteoarthritis: A Comprehensive Review.Biomolecules · 2024Review
- Mechanical stimulation promotes fibrochondrocyte proliferation by activating the TRPV4 signaling pathway during tendon-bone insertion healing: CCN2 plays an important regulatory role.Burns & trauma · 2024Article
- Alterations in the microenvironment and the effects produced of TRPV5 in osteoporosis.Journal of translational medicine · 2023Review
- Mechanosensitive TRPV4 is required for crystal-induced inflammation.Annals of the rheumatic diseases · 2021Article
- Alterations in Anandamide Synthesis and Degradation during Osteoarthritis Progression in an Animal Model.International journal of molecular sciences · 2020Article
- Physiological Effects of the Electrogenic Current Generated by the NaBioelectricity · 2020Article
- TRPV6 as A Target for Cancer Therapy.Journal of Cancer · 2020Review
- Immune aspects of the bi-directional neuroimmune facilitator TRPV1.Molecular biology reports · 2019Review
- Functional Expression of TRP Ion Channels in Endometrial Stromal Cells of Endometriosis Patients.International journal of molecular sciences · 2018Article
- Influence of Mechanical Unloading on Articular Chondrocyte Dedifferentiation.International journal of molecular sciences · 2018Article
- Polymodal Transient Receptor Potential Vanilloid (TRPV) Ion Channels in Chondrogenic Cells.International journal of molecular sciences · 2015Article
- TRPV4 as a therapeutic target for joint diseases.Naunyn-Schmiedeberg's archives of pharmacology · 2015Review
- Changes in Membrane Receptors and Ion Channels as Potential Biomarkers for Osteoarthritis.Frontiers in physiology · 2015Review
- Transforming growth factor beta signaling is essential for the autonomous formation of cartilage-like tissue by expanded chondrocytes.PloS one · 2015Article
- TRPV4-mediated mechanotransduction regulates the metabolic response of chondrocytes to dynamic loading.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ion channels play important roles in chondrocyte mechanotransduction. The transient receptor potential vanilloid (TRPV) subfamily of ion channels consists of six members. TRPV1-4 are temperature sensitive calcium-permeable, relatively non-selective cation channels whereas TRPV5 and TRPV6 show high selectivity for calcium over other cations. In this study we investigated the effect of time in culture and passage number on the expression of TRPV4, TRPV5 and TRPV6 in articular chondrocytes isolated from equine metacarpophalangeal joints. Polyclonal antibodies raised against TRPV4, TRPV5 and TRPV6 were used to compare the expression of these channels in lysates from first expansion chondrocytes (P0) and cells from passages 1-3 (P1, P2 and P3) by western blotting. TRPV4, TRPV5 and TRPV6 were expressed in all passages examined. Immunohistochemistry and immunofluorescence confirmed the presence of these channels in sections of formalin fixed articular cartilage and monolayer cultures of methanol fixed P2 chondrocytes. TRPV5 and TRPV6 were upregulated with time and passage in culture suggesting that a shift in the phenotype of the cells in monolayer culture alters the expression of these channels. In conclusion, several TRPV channels are likely to be involved in calcium signaling and homeostasis in chondrocytes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.