ArticleInternational journal of nanomedicine2012
The role of cholesterol-sphingomyelin membrane nanodomains in the stability of intercellular membrane nanotubes.
Article in International journal of nanomedicine, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 48 citations in OpenAlex.
- Sorting of lysosomal enzyme and autophagy are regulated by the GGA1-induced TGN lipid scrambling.Science advances · 2026Article
- Building the bridges: molecular mechanisms of tunneling nanotube formation.Cellular and molecular life sciences : CMLS · 2026Review
- Breaking cellular boundaries: molecular mechanisms of tunneling nanotube formation and fusion.Biochemical Society transactions · 2026Review
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- Type-1 inflammatory imprinting and programmed responsiveness to CD40L enhance Siglec-1-dependent HIV-1 trans-infection by dendritic cells.Frontiers in immunology · 2026Article
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- UV-B Radiation Disrupts Membrane Lipid Organization and Suppresses Protein Mobility of GmNARK inPlants (Basel, Switzerland) · 2024Article
- Molecular, morphological and functional properties of tunnelling nanotubes between normal and cancer urothelial cells: New insights from theFrontiers in cell and developmental biology · 2022Article
- Peering into tunneling nanotubes-The path forward.The EMBO journal · 2021Review
- Membrane nanotubes are ancient machinery for cell-to-cell communication and transport. Their interference with the immune system.Biologia futura · 2021Review
- Mechanical Principles Governing the Shapes of Dendritic Spines.Frontiers in physiology · 2021Article
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- Modeling membrane nanotube morphology: the role of heterogeneity in composition and material properties.Scientific reports · 2020Article
- Salicylic acid-mediated plasmodesmal closure via Remorin-dependent lipid organization.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Tunneling nanotubes evoke pericyte/endothelial communication during normal and tumoral angiogenesis.Fluids and barriers of the CNS · 2018Article
- The role of traction in membrane curvature generation.Molecular biology of the cell · 2018Article
- The growth determinants and transport properties of tunneling nanotube networks between B lymphocytes.Cellular and molecular life sciences : CMLS · 2016Article
- Linked in: immunologic membrane nanotube networks.Journal of leukocyte biology · 2016Review
- Novel drug-delivery approaches to the blood-brain barrier.Neuroscience bulletin · 2015Review
- Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intercellular membrane nanotubes (ICNs) are highly curved tubular structures that connect neighboring cells. The stability of these structures depends on the inner cytoskeleton and the cell membrane composition. Yet, due to the difficulty in the extraction of ICNs, the cell membrane composition remains elusive. In the present study, a raft marker, ostreolysin, revealed the enrichment of cholesterol-sphingomyelin membrane nanodomains along ICNs in a T24 (malignant) urothelial cancer cell line. Cholesterol depletion, due to the addition of methyl-β-cyclodextrin, caused the dispersion of cholesterol-sphingomyelin membrane nanodomains and the retraction of ICNs. The depletion of cholesterol also led to cytoskeleton reorganization and to formation of actin stress fibers. Live cell imaging data revealed the possible functional coupling between the change from polygonal to spherical shape, cell separation, and the disconnection of ICNs. The ICN was modeled as an axisymmetric tubular structure, enabling us to investigate the effects of cholesterol content on the ICN curvature. The removal of cholesterol was predicted to reduce the positive spontaneous curvature of the remaining membrane components, increasing their curvature mismatch with the tube curvature. The mechanisms by which the increased curvature mismatch could contribute to the disconnection of ICNs are discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.