Evidence map›Paper›PMID 22605481›Full record

ArticleStem cells (Dayton, Ohio)2012

Exosome-mediated transfer of miR-133b from multipotent mesenchymal stromal cells to neural cells contributes to neurite outgrowth.

Hongqi Xin, Yi Li, Ben Buller, Mark Katakowski, Yi Zhang, Xinli Wang, Xia Shang, Zheng Gang Zhang, Michael Chopp

Registry-linked trialOpen access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells (Dayton, Ohio), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03437759 (Mesenchymal Stem Cells Derived Exosomes Promote Healing of Large and Refractory Macular Holes), which is not on this map. Cited by 517 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
517citing papers in PubMed, 2 pooled it
18.9field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT03437759 early_phase1unknown statusnot on this mapstarted 2017, after this paper: background citation

Mesenchymal Stem Cells Derived Exosomes Promote Healing of Large and Refractory Macular Holes

TypeinterventionalSponsorTianjin Medical UniversityRan2017 to 2021Enrolled44ConditionsMacular HolesArmsexosomes derived from mesenchymal stem cells (MSC-Exo)
3 · Its place in the literature

Who cites it

517 citing papers in PubMed, 2 syntheses or guidelines pooled it, 899 citations in OpenAlex.

  1. Pooled it
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  5. Article
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  7. Exosome-mediated siRNA delivery in cancer: Loading strategies, targeting approaches, and therapeutic outcomes.Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences · 2026
    Review
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  9. Article
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457 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Hongqi XinDepartment of Neurology, Henry Ford Hospital, Detroit, MI 48202, USA.
Yi Li
Ben Buller
Mark Katakowski
Yi Zhang
Xinli Wang
Xia Shang
Zheng Gang Zhang
Michael Chopp
Henry Ford Hospital · USOakland University · US

Funding

MicroRNAs and neurogenesis after strokeR01NS075156 · NINDS · HENRY FORD HEALTH SYSTEM · PI ZHANG, ZHENG GANG · 2011 to 2015
$1.6M
tPA, white matter and cell therapy for strokeR01NS066041 · NINDS · HENRY FORD HEALTH SYSTEM · PI LIU, ZHONGWU · 2010 to 2014
$1.6M
MSCs Induce Brain Plasticity via tPAR01AG037506 · NIA · HENRY FORD HEALTH SYSTEM · PI CHOPP, MICHAEL · 2011 to 2015
$1.5M
NIA NIH HHS R01 AG037506NINDS NIH HHS R01 NS066041NINDS NIH HHS R01 NS075156NINDS NIH HHS R01 NS66041NINDS NIH HHS R01 NS75156
6 · The paper itself

Abstract

Multipotent mesenchymal stromal cells (MSCs) have potential therapeutic benefit for the treatment of neurological diseases and injury. MSCs interact with and alter brain parenchymal cells by direct cell-cell communication and/or by indirect secretion of factors and thereby promote functional recovery. In this study, we found that MSC treatment of rats subjected to middle cerebral artery occlusion (MCAo) significantly increased microRNA 133b (miR-133b) level in the ipsilateral hemisphere. In vitro, miR-133b levels in MSCs and in their exosomes increased after MSCs were exposed to ipsilateral ischemic tissue extracts from rats subjected to MCAo. miR-133b levels were also increased in primary cultured neurons and astrocytes treated with the exosome-enriched fractions released from these MSCs. Knockdown of miR-133b in MSCs confirmed that the increased miR-133b level in astrocytes is attributed to their transfer from MSCs. Further verification of this exosome-mediated intercellular communication was performed using a cel-miR-67 luciferase reporter system and an MSC-astrocyte coculture model. Cel-miR-67 in MSCs was transferred to astrocytes via exosomes between 50 and 100 nm in diameter. Our data suggest that the cel-miR-67 released from MSCs was primarily contained in exosomes. A gap junction intercellular communication inhibitor arrested the exosomal microRNA communication by inhibiting exosome release. Cultured neurons treated with exosome-enriched fractions from MSCs exposed to 72 hours post-MCAo brain extracts significantly increased the neurite branch number and total neurite length. This study provides the first demonstration that MSCs communicate with brain parenchymal cells and may regulate neurite outgrowth by transfer of miR-133b to neural cells via exosomes.

Indexed as

AnimalsCells, CulturedExosomesMaleMesenchymal Stem CellsMicroRNAsMicroscopy, Electron, TransmissionNeuritesNeurogenesisNeuronsRatsRats, WistarMicroRNAs

Identifiers

PMID22605481
PMCPMC3495063
OpenAlexW2058180357

What OpenQuestion holds

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Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.