ArticleNucleic acids research2012
Repetitive elements and enforced transcriptional repression co-operate to enhance DNA methylation spreading into a promoter CpG-island.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 32 citations in OpenAlex.
- Characterizing cytosine methylation of polymorphic transposable element insertions using the human pangenome resources.Genome research · 2026Article
- Genome-wide screening and functional validation of methylation barriers near promoters.Nucleic acids research · 2024Article
- Non-pathogenic microbiota accelerate age-related CpG Island methylation in colonic mucosa.Epigenetics · 2023Article
- Myocardial Infarction-Induced INSL6 Decrease Contributes to Breast Cancer Progression.Disease markers · 2023Article
- Characterization of human mitochondrial ferritin promoter: identification of transcription factors and evidences of epigenetic control.Scientific reports · 2016Article
- Imprinting defects at human 14q32 locus alters gene expression and is associated with the pathobiology of osteosarcoma.Oncotarget · 2016Article
- Restricting retrotransposons: a review.Mobile DNA · 2016Review
- The contribution of alu elements to mutagenic DNA double-strand break repair.PLoS genetics · 2015Article
- The Fine LINE: Methylation Drawing the Cancer Landscape.BioMed research international · 2015Review
- Targeted p16(Ink4a) epimutation causes tumorigenesis and reduces survival in mice.The Journal of clinical investigation · 2014Article
- Sequence-level mechanisms of human epigenome evolution.Genome biology and evolution · 2014Article
- Article
- NanoCAGE analysis of the mouse olfactory epithelium identifies the expression of vomeronasal receptors and of proximal LINE elements.Frontiers in cellular neuroscience · 2014Article
- Genome-wide methylation analyses in glioblastoma multiforme.PloS one · 2014Article
- Epigenetic status of LINE-1 predicts clinical outcome in early-stage rectal cancer.British journal of cancer · 2013Article
- CTCF demarcates chicken embryonic α-globin gene autonomous silencing and contributes to adult stage-specific gene expression.Epigenetics · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 5 institutions in 1 country.
Funding
Abstract
Repression of many tumor suppressor genes in cancer is concurrent with aberrantly increased DNA methylation levels at promoter CpG islands (CGIs). About one-fourth of empirically defined human promoters are surrounded by or contain clustered repetitive elements. It was previously observed that a sharp transition of methylation exists between highly methylated repetitive elements and unmethylated promoter-CGIs in normal tissues. The factors that lead to aberrant CGI hypermethylation in cancer remain poorly understood. Here, we established a site-specific integration system with enforced local transcriptional repression in colorectal cancer cells and monitored the occurrence of initial de novo methylation at specific CG sites adjacent to the CGI of the INSL6 promoter, which could be accelerated by binding a KRAB-containing transcriptional factor. Additional repetitive elements from P16 and RIL (PDLIM4), if situated adjacent to the promoter of INSL6, could confer DNA methylation spreading into the CGI particularly in the setting of KRAB-factor binding. However, a repressive chromatin alone was not sufficient to initiate DNA methylation, which required specific DNA sequences and was integration-site (and/or cell-line) specific. Overall, these results demonstrate a requirement for specific DNA sequences to trigger de novo DNA methylation, and repetitive elements as cis-regulatory factors to cooperate with advanced transcriptional repression in promoting methylation spreading.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.