ArticleNature protocols2012
Mechanical fixation techniques for processing and orienting delicate samples, such as the root of Arabidopsis thaliana, for light or electron microscopy.
Article in Nature protocols, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- HMolecular plant pathology · 2025Article
- In-section Click-iT detection and super-resolution CLEM analysis of nucleolar ultrastructure and replication in plants.Nature communications · 2024Article
- Plant mitochondrial FMT and its mammalian homolog CLUH controls development and behavior in Arabidopsis and locomotion in mice.Cellular and molecular life sciences : CMLS · 2022Article
- A non-classical route of efficient plant uptake verified with fluorescent nanoparticles and root adhesion forces investigated using AFM.Scientific reports · 2020Article
- Lactococcus lactis expressing sand fly PpSP15 salivary protein confers long-term protection against Leishmania major in BALB/c mice.PLoS neglected tropical diseases · 2020Article
- Effect of Nanoparticles Surface Charge on theInternational journal of molecular sciences · 2019Article
- bHLH093/NFL and bHLH061 are required for apical meristem function in Arabidopsis thaliana.Plant signaling & behavior · 2018Article
- Embedding and Chemical Reactivation of Green Fluorescent Protein in the Whole Mouse Brain for Optical Micro-Imaging.Frontiers in neuroscience · 2017Article
- Symplastic signaling instructs cell division, cell expansion, and cell polarity in the ground tissue of Arabidopsis thaliana roots.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Ultrastructural Characterization of Turnip Mosaic Virus-Induced Cellular Rearrangements Reveals Membrane-Bound Viral Particles Accumulating in Vacuoles.Journal of virology · 2015Article
- Developing 3D SEM in a broad biological context.Journal of microscopy · 2015Article
- Profile analysis of hepatic porcine and murine brain tissue slices obtained with a vibratome.PeerJ · 2015Article
- Preparation of the planarian Schmidtea mediterranea for high-resolution histology and transmission electron microscopy.Nature protocols · 2014Article
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Authors and funding
3 authors.
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Abstract
Despite improvements in live imaging, fixation followed by embedding and sectioning for light or electron microscopy remains an indispensible approach in biology. During processing, small or delicate samples can be lost, damaged or poorly oriented. Here we present a protocol for overcoming these issues when, along with chemical fixation, the sample is fixed mechanically. The protocol features two alternatives for mechanical fixation: the sample is encased either in a rectangular block of agarose or between Formvar films suspended on a wire loop. We also provide methods for key steps all the way through to sectioning. We illustrate the method on the root of Arabidopsis thaliana, an object that is ∼0.15 mm in diameter and difficult to process conventionally. With this protocol, well-oriented sections can be obtained with excellent ultrastructural preservation. The protocol takes about 1 week.
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