ArticlePathogens and global health2012
Infertility resulting from transgenic I-PpoI male Anopheles gambiae in large cage trials.
Article in Pathogens and global health, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed, 45 citations in OpenAlex.
- Identifying Transgenic Approaches to Mitigate Vector Transmission of Malaria by Anopheles gambiae.Cureus · 2026Review
- Requirements for designing cluster randomised control trials to detect suppression of malaria vector population densities.BMC biology · 2025Article
- Eliminating malaria vectors with precision-guided sterile males.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Eliminating Malaria Vectors with Precision Guided Sterile Males.bioRxiv : the preprint server for biology · 2023Article
- Spatial modelling for population replacement of mosquito vectors at continental scale.PLoS computational biology · 2022Article
- Mark-release-recapture experiment in Burkina Faso demonstrates reduced fitness and dispersal of genetically-modified sterile malaria mosquitoes.Nature communications · 2022Article
- Predicting the spread and persistence of genetically modified dominant sterile male mosquitoes.Parasites & vectors · 2021Article
- CRISPR/Cas9 knockout of female-biased genes AeAct-4 or myo-fem in Ae. aegypti results in a flightless phenotype in female, but not male mosquitoes.PLoS neglected tropical diseases · 2020Article
- Adequacy and sufficiency evaluation of existing EFSA guidelines for the molecular characterisation, environmental risk assessment and post-market environmental monitoring of genetically modified insects containing engineered gene drives.EFSA journal. European Food Safety Authority · 2020Article
- Detecting the population dynamics of an autosomal sex ratio distorter transgene in malaria vector mosquitoes.The Journal of applied ecology · 2020Article
- Progress towards engineering gene drives for population control.The Journal of experimental biology · 2020Review
- Differentially and Co-expressed Genes in Embryo, Germ-Line and Somatic Tissues ofG3 (Bethesda, Md.) · 2019Article
- Plasmodium falciparum (Haemosporodia: Plasmodiidae) and O'nyong-nyong Virus Development in a Transgenic Anopheles gambiae (Diptera: Culicidae) Strain.Journal of medical entomology · 2019Article
- Gene drives to fight malaria: current state and future directions.Pathogens and global health · 2017Review
- Article
- Cheating evolution: engineering gene drives to manipulate the fate of wild populations.Nature reviews. Genetics · 2016Review
- Evidence for temporal population replacement and the signature of ecological adaptation in a major Neotropical malaria vector in Amazonian Peru.Malaria journal · 2015Article
- Stimulating Anopheles gambiae swarms in the laboratory: application for behavioural and fitness studies.Malaria journal · 2015Article
- Modelling sterile insect technique to control the population of Anopheles gambiae.Malaria journal · 2015Article
- Understanding the DNA damage response in order to achieve desired gene editing outcomes in mosquitoes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesAnopheles gambiae is the primary vector of malaria in sub-Saharan Africa and is a potential target of genetic control programs. We determined the capacity of male A. gambiae created by germline transformation to introduce infertility into stable age-distribution populations. We also determined effects of the transgenes on life history.
methodsStable age-distribution populations of A. gambiae mosquitoes were established in large indoor cages. Male mosquitoes carrying an I-PpoI homing endonuclease gene were introduced at ×5 and ×10 release rates where they competed with target male mosquitoes for matings. Similar trials were conducted in small cages with an additional ×1 release level.
resultsInfertility was successfully introduced into all target populations. In supporting experiments, complete female infertility was observed in all strains and species of the A. gambiae complex to which transgenic males were mated. Life history experiments demonstrated that reductions in I-PpoI male vigor exist in the form of reduced adult male emergence, longevity and competitiveness. DISCUSSION: A. gambiae I-PpoI males are capable of introducing high levels of infertility in target populations in indoor cage trials. This was accomplished despite losses of vigor resulting from the HEG transgene. These results motivate further trials of sexually I-PpoI A. gambiae in outdoor cage and field trials.
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