ArticleBiomaterials2012
Controlling the contractile strength of engineered cardiac muscle by hierarchal tissue architecture.
Article in Biomaterials, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 126 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
126 citing papers in PubMed.
- Structural and functional benchmarking of monolayer- and bioreactor-generated hiPSC-derived cardiomyocytes.APL bioengineering · 2026Article
- Restoring anisotropy after myocardial injury: strategies to align transplanted human induced pluripotent stem-cell-derived cardiomyocytes.Experimental & molecular medicine · 2026Review
- Review
- Restoration of structural organization in engineered cardiac microtissues is promoted by cardiomyocyte beating.Communications biology · 2026Article
- Review
- Cardiac myofibril networks induce shear stress.NPJ systems biology and applications · 2026Article
- In vitro sex-specific function-structure relationship in neonatal rat cardiac monolayers.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- In vitro approaches to mimic cardiac mechanical load dynamics for enhancing maturation and disease modelling.Cardiovascular research · 2025Review
- Adaptive response to electrical pulse stimulation is impaired in FSHD myotubes by DUX4 gene network activation.Scientific reports · 2025Article
- Characterizing the Impact of Fabrication Methods on Mechanically Tunable Gelatin Hydrogels for Cardiac Fibrosis Studies.Bioengineering (Basel, Switzerland) · 2025Article
- Micropatterning Photoconductive Peptide Assemblies on Stiff and Soft Biomaterial Substrates.ACS applied materials & interfaces · 2025Article
- Dynamic substrate topographies drive actin- and vimentin-mediated nuclear mechanoprotection events in human fibroblasts.BMC biology · 2025Article
- Harnessing native blueprints for designing bioinks to bioprint functional cardiac tissue.iScience · 2025Review
- Cell-scale porosity minimizes foreign body reaction and promotes innervated myofiber formation after volumetric muscle loss.NPJ Regenerative medicine · 2025Article
- Biomimetic Adaptive Building Façade Modeling for Sustainable Urban Freshwater Ecosystems: Integration of Nature's Water-Harvesting Strategy into Sun-Breakers.Biomimetics (Basel, Switzerland) · 2024Article
- Human Skeletal Muscle Myoblast Culture in Aligned Bacterial Nanocellulose and Commercial Matrices.ACS applied materials & interfaces · 2024Article
- Engineering a robust and anisotropic cardiac-specific extracellular matrix scaffold for cardiac patch tissue engineering.Matrix biology plus · 2024Article
- Engineering programmable material-to-cell pathways via synthetic notch receptors to spatially control differentiation in multicellular constructs.Nature communications · 2024Article
- Reduced Graphene-Oxide-Doped Elastic Biodegradable Polyurethane Fibers for Cardiomyocyte Maturation.ACS biomaterials science & engineering · 2024Article
- Selective Induction of Molecular Assembly to Tissue-Level Anisotropy on Peptide-Based Optoelectronic Cardiac Biointerfaces.Advanced materials (Deerfield Beach, Fla.) · 2024Article
66 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
The heart is a muscular organ with a wrapping, laminar structure embedded with neural and vascular networks, collagen fibrils, fibroblasts, and cardiac myocytes that facilitate contraction. We hypothesized that these non-muscle components may have functional benefit, serving as important structural alignment cues in inter- and intra-cellular organization of cardiac myocytes. Previous studies have demonstrated that alignment of engineered myocardium enhances calcium handling, but how this impacts actual force generation remains unclear. Quantitative assays are needed to determine the effect of alignment on contractile function and muscle physiology. To test this, micropatterned surfaces were used to build 2-dimensional myocardium from neonatal rat ventricular myocytes with distinct architectures: confluent isotropic (serving as the unaligned control), confluent anisotropic, and 20 μm spaced, parallel arrays of multicellular myocardial fibers. We combined image analysis of sarcomere orientation with muscular thin film contractile force assays in order to calculate the peak sarcomere-generated stress as a function of tissue architecture. Here we report that increasing peak systolic stress in engineered cardiac tissues corresponds with increasing sarcomere alignment. This change is larger than would be anticipated from enhanced calcium handling and increased uniaxial alignment alone. These results suggest that boundary conditions (heterogeneities) encoded in the extracellular space can regulate muscle tissue function, and that structural organization and cytoskeletal alignment are critically important for maximizing peak force generation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.