ArticleIn vitro cellular & developmental biology. Plant : journal of the Tissue Culture Association2012
An alginate-layer technique for culture of Brassica oleracea L. protoplasts.
Article in In vitro cellular & developmental biology. Plant : journal of the Tissue Culture Association, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed.
- An effective method for isolation and regeneration of Solanum tuberosum mesophyll protoplasts for transgene-free genome editing.Journal, genetic engineering & biotechnology · 2026Article
- Red and Far-Red LED Lighting Enhances Protoplast-to-Plant Regeneration in Broccoli (Plants (Basel, Switzerland) · 2026Article
- Optimization of protoplast cell division and microcallus formation toward somatic embryogenesis in oil palm.Frontiers in plant science · 2026Article
- Impedance flow cytometry for rapid quality assessment of protoplast cultures.Plant methods · 2025Article
- A novel protocol for protoplast isolation, transfection, and culture in Cannabis sativa L.BMC plant biology · 2025Article
- Demethylating drugs alter protoplast development, regeneration, and the genome stability of protoplast-derived regenerants of cabbage.BMC plant biology · 2025Article
- Development of a highly efficient protoplast regeneration and transfection protocol for enhancing CRISPR genome editing ofFrontiers in plant science · 2025Article
- Development of an optimized protocol for protoplast-to-plant regeneration of selected varieties of Brassica oleracea L.BMC plant biology · 2024Article
- OptimizingPlants (Basel, Switzerland) · 2024Review
- Response surface methodology mediated optimization of phytosulfokine and plant growth regulators for enhanced protoplast division, callus induction, and somatic embryogenesis in Angelica Gigas Nakai.BMC plant biology · 2024Article
- Phytosulfokine alpha enhances regeneration of transformed and untransformed protoplasts ofFrontiers in plant science · 2024Article
- Efficient regeneration of protoplasts fromBiology methods & protocols · 2024Review
- First Report on Mesophyll Protoplast Isolation and Regeneration System for thePlants (Basel, Switzerland) · 2023Article
- Improvements in Protoplast Isolation Protocol and Regeneration of Different Cabbage (Plants (Basel, Switzerland) · 2023Article
- Promotive effect of phytosulfokine - peptide growth factor - on protoplast cultures development in Fagopyrum tataricum (L.) Gaertn.BMC plant biology · 2023Article
- Protoplast Technology and Somatic Hybridisation in the Family Apiaceae.Plants (Basel, Switzerland) · 2023Review
- Optimization of cabbage (Frontiers in plant science · 2023Article
- Overcoming roadblocks forFrontiers in plant science · 2023Review
- Isolation, purification and PEG-mediated transient expression of mesophyll protoplasts in Camellia oleifera.Plant methods · 2022Article
- Optimization of protoplast regeneration in the model plant Arabidopsis thaliana.Plant methods · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ten accessions belonging to the Brassica oleracea subspecies alba and rubra, and to B. oleracea var. sabauda were used in this study. Protoplasts were isolated from leaves and hypocotyls of in vitro grown plants. The influence of selected factors on the yield, viability, and mitotic activity of protoplasts immobilized in calcium alginate layers was investigated. The efficiency of protoplast isolation from hypocotyls was lower (0.7 ± 0.1 × 10(6) ml(-1)) than for protoplasts isolated from leaf mesophyll tissue (2 ± 0.1 × 10(6) ml(-1)). High (70-90%) viabilities of immobilized protoplasts were recorded, independent of the explant sources. The highest proportion of protoplasts undergoing divisions was noted for cv. Reball F1, both from mesophyll (29.8 ± 2.2%) and hypocotyl (17.5 ± 0.3%) tissues. Developed colonies of callus tissue were subjected to regeneration and as a result plants from six accessions were obtained.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.