ArticleThe Journal of biological chemistry2012
Structural and mechanistic studies of pesticin, a bacterial homolog of phage lysozymes.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 62 citations in OpenAlex.
- Unmasking pathogen traits for chronic colonization in neurogenic bladder.Cell reports · 2026Article
- Emergence, global dispersal, and local adaptations ofApplied and environmental microbiology · 2026Review
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- Telomere bacteriophages are widespread and equip their bacterial hosts with potent interbacterial weapons.Science advances · 2025Article
- Trichodesmium metaproteomes reflect the differential influence of resource availability across ocean regions.The ISME journal · 2025Article
- A coordinated attack by a bacterial secretion system and a small molecule drives prey specificity.Communications biology · 2024Article
- Structural constraints of pyocin S2 import through the ferripyoverdine receptor FpvAI.PNAS nexus · 2024Article
- Pesticin-Like Effector VgrG3Microbiology spectrum · 2023Article
- Insights into the genome of Methylobacterium sp. NMS14P, a novel bacterium for growth promotion of maize, chili, and sugarcane.PloS one · 2023Article
- The Type VI Secretion Systems in Plant-Beneficial Bacteria Modulate Prokaryotic and Eukaryotic Interactions in the Rhizosphere.Frontiers in microbiology · 2022Article
- The extracellular contractile injection system is enriched in environmental microbes and associates with numerous toxins.Nature communications · 2021Article
- Antibiotic Therapy of Plague: A Review.Biomolecules · 2021Review
- Targeting the Achilles' Heel of Bacteria: Different Mechanisms To Break Down the Peptidoglycan Cell Wall during Bacterial Warfare.Journal of bacteriology · 2021Review
- Polymorphic Toxins and Their Immunity Proteins: Diversity, Evolution, and Mechanisms of Delivery.Annual review of microbiology · 2020Review
- Comparative genomic analysis of the secondary flagellar (flag-2) system in the order Enterobacterales.BMC genomics · 2020Article
- Origin of a Core Bacterial Gene via Co-option and Detoxification of a Phage Lysin.Current biology : CB · 2019Article
- Structure of the atypical bacteriocin pectocin M2 implies a novel mechanism of protein uptake.Molecular microbiology · 2014Article
- Bacteriophage-based synthetic biology for the study of infectious diseases.Current opinion in microbiology · 2014Article
- Comparative genomics of type VI secretion systems in strains of Pantoea ananatis from different environments.BMC genomics · 2014Article
- Structures of a bifunctional cell wall hydrolase CwlT containing a novel bacterial lysozyme and an NlpC/P60 DL-endopeptidase.Journal of molecular biology · 2014Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Yersinia pestis produces and secretes a toxin named pesticin that kills related bacteria of the same niche. Uptake of the bacteriocin is required for activity in the periplasm leading to hydrolysis of peptidoglycan. To understand the uptake mechanism and to investigate the function of pesticin, we combined crystal structures of the wild type enzyme, active site mutants, and a chimera protein with in vivo and in vitro activity assays. Wild type pesticin comprises an elongated N-terminal translocation domain, the intermediate receptor binding domain, and a C-terminal activity domain with structural analogy to lysozyme homologs. The full-length protein is toxic to bacteria when taken up to the target site via the outer or the inner membrane. Uptake studies of deletion mutants in the translocation domain demonstrate their critical size for import. To further test the plasticity of pesticin during uptake into bacterial cells, the activity domain was replaced by T4 lysozyme. Surprisingly, this replacement resulted in an active chimera protein that is not inhibited by the immunity protein Pim. Activity of pesticin and the chimera protein was blocked through introduction of disulfide bonds, which suggests unfolding as the prerequisite to gain access to the periplasm. Pesticin, a muramidase, was characterized by active site mutations demonstrating a similar but not identical residue pattern in comparison with T4 lysozyme.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.