ArticleMolecular biology and evolution2012
Ultradeep sequencing analysis of population dynamics of virus escape mutants in RNAi-mediated resistant plants.
Article in Molecular biology and evolution, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 42 citations in OpenAlex.
- Targeting of genomic and negative-sense strands of viral RNA contributes to antiviral resistance mediated by artificial miRNAs and promotes the emergence of complex viral populations.Molecular plant pathology · 2022Article
- Innate immune pathways act synergistically to constrain RNA virus evolution in Drosophila melanogaster.Nature ecology & evolution · 2022Article
- Multi-targeting of viral RNAs with synthetic trans-acting small interfering RNAs enhances plant antiviral resistance.The Plant journal : for cell and molecular biology · 2019Article
- Mechanisms and consequences of diversity-generating immune strategies.Nature reviews. Immunology · 2017Review
- swga: a primer design toolkit for selective whole genome amplification.Bioinformatics (Oxford, England) · 2017Article
- Analysis of intra-host genetic diversity of Prunus necrotic ringspot virus (PNRSV) using amplicon next generation sequencing.PloS one · 2017Article
- Inferring the Origin of Metastases from Cancer Phylogenies.Cancer research · 2015Review
- Evaluating the within-host fitness effects of mutations fixed during virus adaptation to different ecotypes of a new host.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Article
- Deep sequencing analysis of viral infection and evolution allows rapid and detailed characterization of viral mutant spectrum.Bioinformatics (Oxford, England) · 2015Article
- Review
- Group Selection and Contribution of Minority Variants during Virus Adaptation Determines Virus Fitness and Phenotype.PLoS pathogens · 2015Article
- Deep sequencing of virus-derived small interfering RNAs and RNA from viral particles shows highly similar mutational landscapes of a plant virus population.Journal of virology · 2015Article
- Distinguishing low frequency mutations from RT-PCR and sequence errors in viral deep sequencing data.BMC genomics · 2015Article
- Biotechnological strategies and tools for Plum pox virus resistance: trans-, intra-, cis-genesis, and beyond.Frontiers in plant science · 2015Review
- Plant miRNAome and antiviral resistance: a retrospective view and prospective challenges.Virus genes · 2014Review
- The role of innate immunity in conditioning mosquito susceptibility to West Nile virus.Viruses · 2013Review
- Non-coding RNAs in crop genetic modification: considerations and predictable environmental risk assessments (ERA).Molecular biotechnology · 2013Review
- Improving the effectiveness of artificial microRNA (amiR)-mediated resistance against Turnip mosaic virus by combining two amiRs or by targeting highly conserved viral genomic regions.Journal of virology · 2013Article
- Article
- Everybody wins! Poland hosts thrilling competitions of viruses, RNAi and football teams.EMBO reports · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 5 institutions in 3 countries.
Funding
Abstract
Plant artificial micro-RNAs (amiRs) have been engineered to target viral genomes and induce their degradation. However, the exceptional evolutionary plasticity of RNA viruses threatens the durability of the resistance conferred by these amiRs. It has recently been shown that viral populations not experiencing strong selective pressure from an antiviral amiR may already contain enough genetic variability in the target sequence to escape plant resistance in an almost deterministic manner. Furthermore, it has also been shown that viral populations exposed to subinhibitory concentrations of the antiviral amiR speed up this process. In this article, we have characterized the molecular evolutionary dynamics of an amiR target sequence in a viral genome under both conditions. The use of Illumina ultradeep sequencing has allowed us to identify virus sequence variants at frequencies as low as 2 × 10(-6) and to track their variation in time before and after the viral population was able of successfully infecting plants fully resistant to the ancestral virus. We found that every site in the amiR-target sequence of the viral genome presented variation and that the variant that eventually broke resistance was sampled among the many coexisting ones. In this system, viral evolution in fully susceptible plants results from an equilibrium between mutation and genetic drift, whereas evolution in partially resistant plants originates from more complex dynamics involving mutation, selection, and drift.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.