ArticleMolecular biology of the cell2012
The Msb3/Gyp3 GAP controls the activity of the Rab GTPases Vps21 and Ypt7 at endosomes and vacuoles.
Article in Molecular biology of the cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 55 citations in OpenAlex.
- The GTPase activating protein Gyp7 regulates Rab7/Ypt7 activity on late endosomes.The Journal of cell biology · 2024Article
- Transport mechanisms between the endocytic, recycling, and biosynthetic pathways via endosomes and theFrontiers in cell and developmental biology · 2024Review
- The yeast endocytic early/sorting compartment exists as an independent sub-compartment within theeLife · 2023Article
- TBC1D18 is a Rab5-GAP that coordinates endosome maturation together with Mon1.The Journal of cell biology · 2022Article
- The MyLO CRISPR-Cas9 toolkit: a markerless yeast localization and overexpression CRISPR-Cas9 toolkit.G3 (Bethesda, Md.) · 2022Article
- The PripA-TbcrA complex-centered Rab GAP cascade facilitates macropinosome maturation in Dictyostelium.Nature communications · 2022Article
- A positive feedback loop involving the Spa2 SHD domain contributes to focal polarization.PloS one · 2022Article
- Arf1 orchestrates Rab GTPase conversion at theMolecular biology of the cell · 2021Article
- Article
- Biogenesis of Extracellular Vesicles.Sub-cellular biochemistry · 2021Article
- Regulation of TORC2 function and localization by Rab5 GTPases inCell cycle (Georgetown, Tex.) · 2019Review
- Rab5 GTPases are required for optimal TORC2 function.The Journal of cell biology · 2019Article
- Rab5-mediated endosome formation is regulated at theCommunications biology · 2019Article
- Rab5-independent activation and function of yeast Rab7-like protein, Ypt7p, in the AP-3 pathway.PloS one · 2019Article
- The Rab GTPase activating protein Gyp2 contributes to UV stress tolerance in Metarhizium acridum.World journal of microbiology & biotechnology · 2018Article
- Regulation of Rab5 isoforms by transcriptional and post-transcriptional mechanisms in yeast.FEBS letters · 2017Article
- Retromer-driven membrane tubulation separates endosomal recycling from Rab7/Ypt7-dependent fusion.Molecular biology of the cell · 2017Article
- Steric hindrance of SNARE transmembrane domain organization impairs the hemifusion-to-fusion transition.EMBO reports · 2016Article
- Polarized Exocytosis Induces Compensatory Endocytosis by Sec4p-Regulated Cortical Actin Polymerization.PLoS biology · 2016Article
- Identification of a Rab GTPase-activating protein cascade that controls recycling of the Rab5 GTPase Vps21 from the vacuole.Molecular biology of the cell · 2015Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Fusion of organelles in the endomembrane system depends on Rab GTPases that interact with tethering factors before lipid bilayer mixing. In yeast, the Rab5 GTPase Vps21 controls fusion and membrane dynamics between early and late endosomes. Here we identify Msb3/Gyp3 as a specific Vps21 GTPase-activating protein (GAP). Loss of Msb3 results in an accumulation of Vps21 and one of its effectors Vps8, a subunit of the CORVET complex, at the vacuole membrane in vivo. In agreement, Msb3 forms a specific transition complex with Vps21, has the highest activity of all recombinant GAPs for Vps21 in vitro, and is found at vacuoles despite its predominant localization to bud tips and bud necks at the plasma membrane. Surprisingly, Msb3 also inhibits vacuole fusion, which can be rescued by the Ypt7 GDP-GTP exchange factor (GEF), the Mon1-Ccz1 complex. Consistently, msb3 vacuoles fuse more efficiently than wild-type vacuoles in vitro, suggesting that GAP can also act on Ypt7. Our data indicate that GAPs such as Msb3 can act on multiple substrates in vivo at both ends of a trafficking pathway. This ensures specificity of the subsequent GEF-mediated activation of the Rab that initiates the next transport event.
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