ArticleMolecular & cellular proteomics : MCP2012
Chemical visualization of phosphoproteomes on membrane.
Article in Molecular & cellular proteomics : MCP, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Phosphatase specificity principles uncovered by MRBLE:Dephos and global substrate identification.Molecular systems biology · 2023Article
- Identification of Novel Kinases of Tau Using Fluorescence Complementation Mass Spectrometry (FCMS).Molecular & cellular proteomics : MCP · 2022Article
- Hip2 ubiquitin-conjugating enzyme has a role in UV-induced G1/S arrest and re-entry.Genes & genomics · 2019Article
- Plk1-Mediated Phosphorylation of TSC1 Enhances the Efficacy of Rapamycin.Cancer research · 2018Article
- Plk1 Phosphorylation of Mre11 Antagonizes the DNA Damage Response.Cancer research · 2017Article
- Phosphoproteins in extracellular vesicles as candidate markers for breast cancer.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Multiplexed Imaging of Protein Phosphorylation on Membranes Based on Ti(IV) Functionalized Nanopolymers.Chembiochem : a European journal of chemical biology · 2016Article
- Cotargeting Polo-Like Kinase 1 and the Wnt/β-Catenin Signaling Pathway in Castration-Resistant Prostate Cancer.Molecular and cellular biology · 2015Article
- Over 2300 phosphorylated peptide identifications with single-shot capillary zone electrophoresis-tandem mass spectrometry in a 100 min separation.Analytical chemistry · 2015Article
- Sensitive measurement of total protein phosphorylation level in complex protein samples.The Analyst · 2015Article
- Universal non-antibody detection of protein phosphorylation using pIMAGO.Current protocols in chemical biology · 2015Article
- Identification of rictor as a novel substrate of Polo-like kinase 1.Cell cycle (Georgetown, Tex.) · 2015Article
- Plk1 phosphorylation of PTEN causes a tumor-promoting metabolic state.Molecular and cellular biology · 2014Article
- Specific visualization and identification of phosphoproteome in gels.Analytical chemistry · 2014Article
- Is phosphoproteomics ready for clinical research?Clinica chimica acta; international journal of clinical chemistry · 2013Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
With new discoveries of important roles of phosphorylation on a daily basis, phospho-specific antibodies, as the primary tool for on-membrane detection of phosphoproteins, face enormous challenges. To address an urgent need for convenient and reliable analysis of phosphorylation events, we report a novel strategy for sensitive phosphorylation analysis in the Western blotting format. The chemical reagent, which we termed pIMAGO, is based on a multifunctionalized soluble nanopolymer and is capable of selectively binding to phosphorylated residues independent of amino acid microenvironment, thus offering great promise as a universal tool in biological analyses where the site of phosphorylation is not known or its specific antibody is not available. The specificity and sensitivity of the approach was first examined using a mixture of standard proteins. The method was then applied to monitor phosphorylation changes in in vitro kinase and phosphatase assays. Finally, to demonstrate the unique ability of pIMAGO to measure endogenous phosphorylation, we used it to visualize and determine the differences in phosphorylated proteins that interact with wild-type and kinase dead mutant of Polo-like kinase 1 during mitosis, the results of which were further confirmed by a quantitative phosphoproteomics experiment.
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Registered trials
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