ArticlePlant molecular biology2012
Cloning, functional characterization and genomic organization of 1,8-cineole synthases from Lavandula.
Article in Plant molecular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 69 citations in OpenAlex.
- Rational Enzyme Modification and Expression Enhancement Strategies for 1,8-cineole Production in Serratia marcescens Cell Factories.Applied biochemistry and biotechnology · 2026Article
- The comprehensive review of eucalyptol: synthesis, metabolism, and therapeutic applications in disease treatment.Molecular biology reports · 2025Review
- Impact of drought stress on biochemical and molecular responses in lavender (Frontiers in plant science · 2025Article
- Metabolic engineering of terpene metabolism in lavender.Beni-Suef University journal of basic and applied sciences · 2024Review
- Terpene produced by coexpression of the TPS and P450 genes from Lavandula angustifolia protects plants from herbivore attacks during budding stages.BMC plant biology · 2023Article
- Decoupling subgenomes within hybrid lavandin provide new insights into speciation and monoterpenoid diversification of Lavandula.Plant biotechnology journal · 2023Article
- Lavandula Species, Their Bioactive Phytochemicals, and Their Biosynthetic Regulation.International journal of molecular sciences · 2023Review
- Biochemical basis for the formation of organ-specific volatile blends in mint.Frontiers in plant science · 2023Article
- Chromosome-level genome assembly of Mentha longifolia L. reveals gene organization underlying disease resistance and essential oil traits.G3 (Bethesda, Md.) · 2022Article
- Kinetic studies and homology modeling of a dual-substrate linalool/nerolidol synthase from Plectranthus amboinicus.Scientific reports · 2021Article
- Plasticity engineering of plant monoterpene synthases and application for microbial production of monoterpenoids.Biotechnology for biofuels · 2021Review
- Isoprenoid Metabolism and Engineering in Glandular Trichomes ofFrontiers in plant science · 2021Review
- Short-chain isoprenyl diphosphate synthases of lavender (Lavandula).Plant molecular biology · 2020Article
- Cloning and functional characterization of a floral repressor gene from Lavandula angustifolia.Planta · 2020Article
- Quick and efficient approach to develop genomic resources in orphan species: Application in Lavandula angustifolia.PloS one · 2020Article
- Diverse transcription factors control monoterpene synthase expression in lavender (Lavandula).Planta · 2019Article
- Volatile composition and classification ofHorticulture research · 2019Article
- Article
- Article
- RNA-Seq in the discovery of a sparsely expressed scent-determining monoterpene synthase in lavender (Lavandula).Planta · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several members of the genus Lavandula produce valuable essential oils (EOs) that are primarily constituted of the low molecular weight isoprenoids, particularly monoterpenes. We isolated over 8,000 ESTs from the glandular trichomes of L. x intermedia flowers (where bulk of the EO is synthesized) to facilitate the discovery of genes that control the biosynthesis of EO constituents. The expression profile of these ESTs in L. x intermedia and its parents L. angustifolia and L. latifolia was established using microarrays. The resulting data highlighted a differentially expressed, previously uncharacterized cDNA with strong homology to known 1,8-cineole synthase (CINS) genes. The ORF, excluding the transit peptide, of this cDNA was expressed in E. coli, purified by Ni-NTA agarose affinity chromatography and functionally characterized in vitro. The ca. 63 kDa bacterially produced recombinant protein, designated L. x intermedia CINS (LiCINS), converted geranyl diphosphate (the linear monoterpene precursor) primarily to 1,8-cineole with K ( m ) and k ( cat ) values of 5.75 μM and 8.8 × 10(-3) s(-1), respectively. The genomic DNA of CINS in the studied Lavandula species had identical exon-intron architecture and coding sequences, except for a single polymorphic nucleotide in the L. angustifolia ortholog which did not alter protein function. Additional nucleotide variations restricted to L. angustifolia introns were also observed, suggesting that LiCINS was most likely inherited from L. latifolia. The LiCINS mRNA levels paralleled the 1,8-cineole content in mature flowers of the three lavender species, and in developmental stages of L. x intermedia inflorescence indicating that the production of 1,8 cineole in Lavandula is most likely controlled through transcriptional regulation of LiCINS.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.