ArticlePLoS neglected tropical diseases2012
Proteomic analysis of the cyst stage of Entamoeba histolytica.
Article in PLoS neglected tropical diseases, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 42 citations in OpenAlex.
- Diversity and Plasticity of Virulent Characteristics ofTropical medicine and infectious disease · 2023Review
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- Encystation ofMicroorganisms · 2021Article
- Entamoeba stage conversion: progress and new insights.Current opinion in microbiology · 2020Review
- Proteomic Insights into the Biology of the Most Important Foodborne Parasites in Europe.Foods (Basel, Switzerland) · 2020Review
- Acetylcholine UpregulatesFrontiers in cellular and infection microbiology · 2020Article
- Revisiting Drug Development Against the Neglected Tropical Disease, Amebiasis.Frontiers in cellular and infection microbiology · 2020Review
- Identification and Characterization of the Entamoeba Histolytica Rab8a Binding Protein: A Cdc50 Homolog.International journal of molecular sciences · 2018Article
- Utilization of Different Omic Approaches to Unravel Stress Response Mechanisms in the ParasiteFrontiers in cellular and infection microbiology · 2018Review
- Influence of Micropatterned Grill Lines onFrontiers in cellular and infection microbiology · 2018Article
- Membrane Trafficking Modulation during Entamoeba Encystation.Scientific reports · 2017Article
- Proteome analysis of excretory-secretory proteins ofClinical proteomics · 2016Article
- Proteomic Study of Entamoeba histolytica Trophozoites, Cysts, and Cyst-Like Structures.PloS one · 2016Article
- Methodology for preservation of yeast-bound single chain fragment variable antibody affinity reagents.Journal of immunological methods · 2015Article
- Entamoeba histolytica: protein arginine transferase 1a methylates arginine residues and potentially modify the H4 histone.Parasites & vectors · 2015Article
- Article
- Duplex microfluidic SERS detection of pathogen antigens with nanoyeast single-chain variable fragments.Analytical chemistry · 2014Article
- Strategies to discover the structural components of cyst and oocyst walls.Eukaryotic cell · 2013Review
- The genome and transcriptome of the enteric parasite Entamoeba invadens, a model for encystation.Genome biology · 2013Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundThe category B agent of bioterrorism, Entamoeba histolytica has a two-stage life cycle: an infective cyst stage, and an invasive trophozoite stage. Due to our inability to effectively induce encystation in vitro, our knowledge about the cyst form remains limited. This also hampers our ability to develop cyst-specific diagnostic tools.
aimsThree main aims were (i) to identify E. histolytica proteins in cyst samples, (ii) to enrich our knowledge about the cyst stage, and (iii) to identify candidate proteins to develop cyst-specific diagnostic tools.
methodsCysts were purified from the stool of infected individuals using Percoll (gradient) purification. A highly sensitive LC-MS/MS mass spectrometer (Orbitrap) was used to identify cyst proteins.
resultsA total of 417 non-redundant E. histolytica proteins were identified including 195 proteins that were never detected in trophozoite-derived proteomes or expressed sequence tag (EST) datasets, consistent with cyst specificity. Cyst-wall specific glycoproteins Jacob, Jessie and chitinase were positively identified. Antibodies produced against Jacob identified cysts in fecal specimens and have potential utility as a diagnostic reagent. Several protein kinases, small GTPase signaling molecules, DNA repair proteins, epigenetic regulators, and surface associated proteins were also identified. Proteins we identified are likely to be among the most abundant in excreted cysts, and therefore show promise as diagnostic targets. MAJOR
conclusionsThe proteome data generated here are a first for naturally-occurring E. histolytica cysts, and they provide important insights into the infectious cyst form. Additionally, numerous unique candidate proteins were identified which will aid the development of new diagnostic tools for identification of E. histolytica cysts.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.