ArticlePloS one2012
Chitin binding proteins act synergistically with chitinases in Serratia proteamaculans 568.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Site-directed mutagenesis and semi-rational design to enhance chitinolytic activity of bacterial exochitinase.Microbial cell factories · 2026Article
- Microbial chitinases and their relevance in various industries.Folia microbiologica · 2023Review
- Comparative genomics ofFrontiers in microbiology · 2023Article
- Genetic Determinants of Antagonistic Interactions and the Response of New Endophytic StrainInternational journal of molecular sciences · 2022Article
- Molecular analysis of genes involved in chitin degradation from the chitinolytic bacterium Bacillus velezensis.Antonie van Leeuwenhoek · 2022Article
- Formation of recombinant bifunctional fusion protein: A newer approach to combine the activities of two enzymes in a single protein.PloS one · 2022Article
- A bottom-up approach towards a bacterial consortium for the biotechnological conversion of chitin to L-lysine.Applied microbiology and biotechnology · 2021Article
- The chitinolytic activity of the Curtobacterium sp. isolated from field-grown soybean and analysis of its genome sequence.PloS one · 2021Article
- Chitin and Its Effects on Inflammatory and Immune Responses.Clinical reviews in allergy & immunology · 2018Review
- Genome mining of Streptomyces scabrisporus NF3 reveals symbiotic features including genes related to plant interactions.PloS one · 2018Article
- Article
- Physiological and Molecular Understanding of Bacterial Polysaccharide Monooxygenases.Microbiology and molecular biology reviews : MMBR · 2017Review
- Structure prediction and network analysis of chitinases from the Cape sundew, Drosera capensis.Biochimica et biophysica acta. General subjects · 2017Article
- Chitinase-producing bacteria and their role in biocontrol.AIMS microbiology · 2017Review
- Evidence for an Opportunistic and Endophytic Lifestyle of the Bursaphelenchus xylophilus-Associated Bacteria Serratia marcescens PWN146 Isolated from Wilting Pinus pinaster.Microbial ecology · 2016Article
- Antifungal performance of extracellular chitinases and culture supernatants of Streptomyces galilaeus CFFSUR-B12 against Mycosphaerella fijiensis Morelet.World journal of microbiology & biotechnology · 2016Article
- Comparative genome analyses of Serratia marcescens FS14 reveals its high antagonistic potential.PloS one · 2015Article
- Catalytic efficiency of chitinase-D on insoluble chitinous substrates was improved by fusing auxiliary domains.PloS one · 2015Article
- Cooperative degradation of chitin by extracellular and cell surface-expressed chitinases from Paenibacillus sp. strain FPU-7.Applied and environmental microbiology · 2013Article
- Article
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4 authors.
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Abstract
Genome sequence of Serratia proteamaculans 568 revealed the presence of three family 33 chitin binding proteins (CBPs). The three Sp CBPs (Sp CBP21, Sp CBP28 and Sp CBP50) were heterologously expressed and purified. Sp CBP21 and Sp CBP50 showed binding preference to β-chitin, while Sp CBP28 did not bind to chitin and cellulose substrates. Both Sp CBP21 and Sp CBP50 were synergistic with four chitinases from S. proteamaculans 568 (Sp ChiA, Sp ChiB, Sp ChiC and Sp ChiD) in degradation of α- and β-chitin, especially in the presence of external electron donor (reduced glutathione). Sp ChiD benefited most from Sp CBP21 or Sp CBP50 on α-chitin, while Sp ChiB and Sp ChiD had major advantage with these Sp CBPs on β-chitin. Dose responsive studies indicated that both the Sp CBPs exhibit synergism ≥ 0.2 µM. The addition of both Sp CBP21 and Sp CBP50 in different ratios to a synergistic mixture did not significantly increase the activity. Highly conserved polar residues, important in binding and activity of CBP21 from S. marcescens (Sm CBP21), were present in Sp CBP21 and Sp CBP50, while Sp CBP28 had only one such polar residue. The inability of Sp CBP28 to bind to the test substrates could be attributed to the absence of important polar residues.
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