ArticlePLoS pathogens2012
Large-scale gene disruption in Magnaporthe oryzae identifies MC69, a secreted protein required for infection by monocot and dicot fungal pathogens.
Article in PLoS pathogens, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers.
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Who cites it
84 citing papers in PubMed, 151 citations in OpenAlex.
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- Glycoside Hydrolase Family 16 Enzyme RsEG146 From Rhizoctonia solani AG1 IA Induces Cell Death and Triggers Defence Response in Nicotiana tabacum.Molecular plant pathology · 2025Article
- Fungal evasion ofProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Zinc-finger (ZiF) fold secreted effectors form a functionally diverse family across lineages of the blast fungus Magnaporthe oryzae.PLoS pathogens · 2024Article
- PUFA-PLs biosynthesis enzymes contribute to pathogenic development of rice blast fungusMycology · 2024Article
- Recent Advances in Effector Research ofBiomolecules · 2023Review
- Progress in pathogenesis research of Ustilago maydis, and the metabolites involved along with their biosynthesis.Molecular plant pathology · 2023Review
- The Devastating Rice Blast Airborne PathogenPathogens (Basel, Switzerland) · 2023Review
- The Conserved Cysteine-Rich Secretory Protein MaCFEM85 Interacts with MsWAK16 to Activate Plant Defenses.International journal of molecular sciences · 2023Article
- Screening of Candidate Effectors fromInternational journal of molecular sciences · 2023Article
- Magnaporthe oryzae pathotype Triticum (MoT) can act as a heterologous expression system for fungal effectors with high transcript abundance in wheat.Scientific reports · 2023Article
- Functional Analyses of a Small Secreted Cysteine-Rich Protein ThSCSP_14 inInternational journal of molecular sciences · 2022Article
- Article
- ThSCSP_12: Novel Effector inInternational journal of molecular sciences · 2022Article
- Fungal Effectoromics: A World in Constant Evolution.International journal of molecular sciences · 2022Review
- Advancement in the Breeding, Biotechnological and Genomic Tools towards Development of Durable Genetic Resistance against the Rice Blast Disease.Plants (Basel, Switzerland) · 2022Review
- Functional Identification ofBiomolecules · 2022Article
- Two Magnaporthe appressoria-specific (MAS) proteins, MoMas3 and MoMas5, are required for suppressing host innate immunity and promoting biotrophic growth in rice cells.Molecular plant pathology · 2022Article
- Functional Characterization of the M36 Metalloprotease FgFly1 inJournal of fungi (Basel, Switzerland) · 2022Article
24 more citing papers are in PubMed but not listed here.
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Authors and funding
15 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To search for virulence effector genes of the rice blast fungus, Magnaporthe oryzae, we carried out a large-scale targeted disruption of genes for 78 putative secreted proteins that are expressed during the early stages of infection of M. oryzae. Disruption of the majority of genes did not affect growth, conidiation, or pathogenicity of M. oryzae. One exception was the gene MC69. The mc69 mutant showed a severe reduction in blast symptoms on rice and barley, indicating the importance of MC69 for pathogenicity of M. oryzae. The mc69 mutant did not exhibit changes in saprophytic growth and conidiation. Microscopic analysis of infection behavior in the mc69 mutant revealed that MC69 is dispensable for appressorium formation. However, mc69 mutant failed to develop invasive hyphae after appressorium formation in rice leaf sheath, indicating a critical role of MC69 in interaction with host plants. MC69 encodes a hypothetical 54 amino acids protein with a signal peptide. Live-cell imaging suggested that fluorescently labeled MC69 was not translocated into rice cytoplasm. Site-directed mutagenesis of two conserved cysteine residues (Cys36 and Cys46) in the mature MC69 impaired function of MC69 without affecting its secretion, suggesting the importance of the disulfide bond in MC69 pathogenicity function. Furthermore, deletion of the MC69 orthologous gene reduced pathogenicity of the cucumber anthracnose fungus Colletotrichum orbiculare on both cucumber and Nicotiana benthamiana leaves. We conclude that MC69 is a secreted pathogenicity protein commonly required for infection of two different plant pathogenic fungi, M. oryzae and C. orbiculare pathogenic on monocot and dicot plants, respectively.
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