ArticleThe Plant cell2012
A membrane microdomain-associated protein, Arabidopsis Flot1, is involved in a clathrin-independent endocytic pathway and is required for seedling development.
Article in The Plant cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 93 papers.
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Who cites it
93 citing papers in PubMed, 211 citations in OpenAlex.
- SRF3 couples cellular iron homeostasis and defense responses through ubiquitin-dependent nano-organization.Science advances · 2026Article
- Clathrin-Mediated Endocytosis in Plants: Historical to Modern Advances.Traffic (Copenhagen, Denmark) · 2026Review
- Plasma membrane invaginations and their diverse cellular functions.BMC biology · 2026Review
- Mechanisms controlling the plasma membrane targeting and the nanodomain organization of the plant SPFH protein HIR2.The Plant journal : for cell and molecular biology · 2026Article
- Polymerization-mediated SRFR1 condensation in upper lateral root cap cells regulates root growth.The Plant cell · 2026Article
- Spatiotemporal dynamics of AHA2 reveal that RALF1 induces its endocytosis and vacuolar degradation.Plant communications · 2025Article
- Brassinosteroid Signaling Dynamics: Ubiquitination-Dependent Regulation of Core Signaling Components.International journal of molecular sciences · 2025Review
- Mapping Small Extracellular Vesicle Secretion Potential in Healthy Human Gingiva Using Spatial Transcriptomics.Current issues in molecular biology · 2025Article
- Membrane nanodomains to shape plant cellular functions and signaling.The New phytologist · 2025Review
- Mechanistic divergences of endocytic clathrin-coated vesicle formation in mammals, yeasts and plants.Journal of cell science · 2024Review
- FERONIA homologs in stress responses of horticultural plants: current knowledge and missing links.Stress biology · 2024Review
- Plasmodesmata-associated Flotillin positively regulates broad-spectrum virus cell-to-cell trafficking.Plant biotechnology journal · 2024Article
- A century journey of organelles research in the plant endomembrane system.The Plant cell · 2024Review
- RinRK1 enhances NF receptors accumulation in nanodomain-like structures at root-hair tip.Nature communications · 2024Article
- Role of the dynamin-related protein 2 family and SH3P2 in clathrin-mediated endocytosis in Arabidopsis thaliana.Journal of cell science · 2024Article
- Fungal small RNAs ride in extracellular vesicles to enter plant cells through clathrin-mediated endocytosis.Nature communications · 2023Article
- Fungal small RNAs ride in extracellular vesicles to enter plant cells through clathrin-mediated endocytosis.bioRxiv : the preprint server for biology · 2023Article
- Clathrin-mediated endocytosis facilitates the internalization of Magnaporthe oryzae effectors into rice cells.The Plant cell · 2023Article
- Confocal Microscopy Investigations of Biopolymeric PLGA Nanoparticle Uptake inPlants (Basel, Switzerland) · 2023Article
- Tomato receptor-like cytoplasmic kinase Fir1 is involved in flagellin signaling and preinvasion immunity.Plant physiology · 2023Article
33 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
11 authors at 6 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Endocytosis is essential for the maintenance of protein and lipid compositions in the plasma membrane and for the acquisition of materials from the extracellular space. Clathrin-dependent and -independent endocytic processes are well established in yeast and animals; however, endocytic pathways involved in cargo internalization and intracellular trafficking remain to be fully elucidated for plants. Here, we used transgenic green fluorescent protein-flotillin1 (GFP-Flot1) Arabidopsis thaliana plants in combination with confocal microscopy analysis and transmission electron microscopy immunogold labeling to study the spatial and dynamic aspects of GFP-Flot1-positive vesicle formation. Vesicle size, as outlined by the gold particles, was ∼100 nm, which is larger than the 30-nm size of clathrin-coated vesicles. GFP-Flot1 also did not colocalize with clathrin light chain-mOrange. Variable-angle total internal reflection fluorescence microscopy also revealed that the dynamic behavior of GFP-Flot1-positive puncta was different from that of clathrin light chain-mOrange puncta. Furthermore, disruption of membrane microdomains caused a significant alteration in the dynamics of Flot1-positive puncta. Analysis of artificial microRNA Flot1 transgenic Arabidopsis lines established that a reduction in Flot1 transcript levels gave rise to a reduction in shoot and root meristem size plus retardation in seedling growth. Taken together, these findings support the hypothesis that, in plant cells, Flot1 is involved in a clathrin-independent endocytic pathway and functions in seedling development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.