ArticleJournal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research2012
Villin promoter-mediated transgenic expression of transient receptor potential cation channel, subfamily V, member 6 (TRPV6) increases intestinal calcium absorption in wild-type and vitamin D receptor knockout mice.
Article in Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 74 citations in OpenAlex.
- Disruption of Claudin-2 and Trpv6 Results in Hypocalcemia, Hypercalciuria, and Altered Bone Microarchitecture in Mice.Acta physiologica (Oxford, England) · 2026Article
- The impact of inducible-whole body or intestine-specific Cyp24a1 gene knockout on vitamin D metabolism in mice.The Journal of steroid biochemistry and molecular biology · 2025Article
- Biology of calcium homeostasis regulation in intestine and kidney.Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association · 2025Review
- The role of transient receptor potential channels in chronic kidney disease-mineral and bone disorder.Frontiers in pharmacology · 2025Review
- Male Lrp5A214V mice maintain high bone mass during dietary calcium restriction by altering the vitamin D endocrine system.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2024Article
- Review
- Mineral Supplements in Ageing.Sub-cellular biochemistry · 2024Review
- Maternal Epidermal Growth Factor Promotes Neonatal Claudin-2 Dependent Increases in Small Intestinal Calcium Permeability.Function (Oxford, England) · 2023Article
- Vitamin D-Mediated Regulation of Intestinal Calcium Absorption.Nutrients · 2022Review
- Calcium selective channel TRPV6: Structure, function, and implications in health and disease.Gene · 2022Review
- Vitamin D and Gut Health.Advances in experimental medicine and biology · 2022Article
- TRPV1 channels as a newly identified target for vitamin D.Channels (Austin, Tex.) · 2021Review
- Vitamin D: A Critical Regulator of Intestinal Physiology.JBMR plus · 2021Review
- Probing the Scope and Mechanisms of Calcitriol Actions Using Genetically Modified Mouse Models.JBMR plus · 2021Article
- Quercetin Regulates Calcium and Phosphorus Metabolism Through the Wnt Signaling Pathway in Broilers.Frontiers in veterinary science · 2021Article
- Vitamin D and the intestine: Review and update.The Journal of steroid biochemistry and molecular biology · 2020Review
- Ionomycin ameliorates hypophosphatasia via rescuing alkaline phosphatase deficiency-mediated L-type CaBone research · 2020Article
- Efficacy of 1-α-Hydroxycholecalciferol Supplementation in Young Broiler Feed Suggests Reducing Calcium Levels at Grower Phase.Frontiers in veterinary science · 2020Article
- New developments in our understanding of vitamin metabolism, action and treatment.Metabolism: clinical and experimental · 2019Review
- Activation of the calcium sensing receptor attenuates TRPV6-dependent intestinal calcium absorption.JCI insight · 2019Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Transient receptor potential cation channel, subfamily V, member 6 (TRPV6) is an apical membrane calcium (Ca) channel in the small intestine proposed to be essential for vitamin D-regulated intestinal Ca absorption. Recent studies have challenged the proposed role for TRPV6 in Ca absorption. We directly tested intestinal TRPV6 function in Ca and bone metabolism in wild-type (WT) and vitamin D receptor knockout (VDRKO) mice. TRPV6 transgenic mice (TG) were made with intestinal epithelium-specific expression of a 3X Flag-tagged human TRPV6 protein. TG and VDRKO mice were crossed to make TG-VDRKO mice. Ca and bone metabolism was examined in WT, TG, VDRKO, and TG-VDRKO mice. TG mice developed hypercalcemia and soft tissue calcification on a chow diet. In TG mice fed a 0.25% Ca diet, Ca absorption was more than three-fold higher and femur bone mineral density (BMD) was 26% higher than WT. Renal 1α hydroxylase (CYP27B1) mRNA and intestinal expression of the natural mouse TRPV6 gene were reduced to <10% of WT but small intestine calbindin-D(9k) expression was elevated >15 times in TG mice. TG-VDRKO mice had high Ca absorption that prevented the low serum Ca, high renal CYP27B1 mRNA, low BMD, and abnormal bone microarchitecture seen in VDRKO mice. In addition, small intestinal calbindin D(9K) mRNA and protein levels were elevated in TG-VDRKO. Transgenic TRPV6 expression in intestine is sufficient to increase Ca absorption and bone density, even in VDRKO mice. VDR-independent upregulation of intestinal calbindin D(9k) in TG-VDRKO suggests this protein may buffer intracellular Ca during Ca absorption. © 2012 American Society for Bone and Mineral Research.
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Registered trials
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