ReviewTheory in biosciences = Theorie in den Biowissenschaften2012
Reference genes for measuring mRNA expression.
Review in Theory in biosciences = Theorie in den Biowissenschaften, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
25 citing papers in PubMed.
- Unlocking the Future of Hepatocellular Carcinoma Early Diagnosis: The Promise of Extracellular Vesicle Biomarkers.Journal of clinical and translational hepatology · 2026Review
- Reference Genes for Expression Analyses by qRT-PCR inBiology · 2025Article
- Stabilizing the Baseline: Reference Gene Evaluation in Three InvasiveInternational journal of molecular sciences · 2025Article
- Sex-Specific ADNP/NAP (Davunetide) Regulation of Cocaine-Induced Plasticity.Journal of molecular neuroscience : MN · 2024Article
- Commentary: Identification of optimal reference genes for gene expression normalization in human osteosarcoma cell lines under proliferative conditions.Frontiers in genetics · 2024Article
- Enhanced Nerve Regeneration by Exosomes Secreted by Adipose-Derived Stem Cells with or without FK506 Stimulation.International journal of molecular sciences · 2021Article
- Monogenic Diabetes and Integrated Stress Response Genes Display Altered Gene Expression in Type 1 Diabetes.Diabetes · 2021Article
- Transcriptome Reveals Roles of Lignin-Modifying Enzymes and Abscisic Acid in the Symbiosis ofInternational journal of molecular sciences · 2021Article
- Transcriptomics Curation of SARS-CoV-2 Related Host Genes in Mice With COVID-19 Comorbidity: A Pilot Study.Infectious microbes & diseases · 2020Article
- Validation of Reference Genes for Normalization of Relative qRT-PCR Studies in Papillary Thyroid Carcinoma.Scientific reports · 2019Article
- Identification of stable senescence-associated reference genes.Aging cell · 2019Article
- Reliable reference genes for expression analysis of proliferating and adipogenically differentiating human adipose stromal cells.Cellular & molecular biology letters · 2019Article
- Plant reference genes for development and stress response studies.Journal of biosciences · 2018Review
- Article
- The effects of moderate intensity training in a hypoxic environment on transcriptional responses in Thoroughbred horses.Biology open · 2017Article
- Normalization for Relative Quantification of mRNA and microRNA in Soybean Exposed to Various Abiotic Stresses.PloS one · 2016Article
- Tumor profiling of co-regulated receptor tyrosine kinase and chemoresistant genes reveal different targeting options for lung and gastroesophageal cancers.American journal of translational research · 2016Article
- Reference genes for reverse transcription quantitative PCR in canine brain tissue.BMC research notes · 2015Article
- Selection of suitable reference genes for expression analysis in human glioma using RT-qPCR.Journal of neuro-oncology · 2015Article
- Delineation of gastric cancer subtypes by co-regulated expression of receptor tyrosine kinases and chemosensitivity genes.American journal of translational research · 2015Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this review is to find answers to some of the questions surrounding reference genes and their reliability for quantitative experiments. Reference genes are assumed to be at a constant expression level, over a range of conditions such as temperature. These genes, such as GADPH and beta-actin, are used extensively for gene expression studies using techniques like quantitative PCR. There have been several studies carried out on identifying reference genes. However, a lot of evidence indicates issues to the general suitability of these genes. Recent studies had shown that different factors, including the environment and methods, play an important role in changing the expression levels of the reference genes. Thus, we conclude that there is no reference gene that can deemed suitable for all the experimental conditions. In addition, we believe that every experiment will require the scientific evaluation and selection of the best candidate gene for use as a reference gene to obtain reliable scientific results.
Indexed as
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22588998What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.