ArticleActa biomaterialia2012
Crosslinking and composition influence the surface properties, mechanical stiffness and cell reactivity of collagen-based films.
Article in Acta biomaterialia, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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Who cites it
70 citing papers in PubMed, 245 citations in OpenAlex.
- Cross-Linkable Protein Nanofiber Aerogels for the Removal of Cationic and Anionic Dyes.ACS materials Au · 2026Article
- Animal-Free Derived Collagen-Like Protein Based Electrospun Nanofibers for Biomedical Applications: Cell Interactions Studies.Macromolecular bioscience · 2026Article
- Development of an Active Crosslinked Nanocomposite Film Based on Gelatin-Ethyl Cellulose, Trans-Cinnamaldehyde, and Silver Nanoparticles for the Preservation of Sliced Meat.Journal of food science · 2026Article
- Glyoxalase-1 overexpression attenuates arterial wall stiffening in diabetic mice.Cardiovascular diabetology · 2025Article
- Enhancing Skeletal Muscle Fiber Type Transition Through Substrate Coating Alteration in Myoblast Cell Culture.International journal of molecular sciences · 2025Article
- Functionalization of Two-Component Gelatinous Peptide/Reactive Oligomer Hydrogels with Small Molecular Amines for Enhanced Cellular Interaction.International journal of molecular sciences · 2025Article
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- Enhancing Form Stability: Shrink-Resistant Hydrogels Made of Interpenetrating Networks of Recombinant Spider Silk and Collagen-I.Advanced healthcare materials · 2025Article
- Interpenetrating networks of fibrillar and amorphous collagen promote cell spreading and hydrogel stability.Acta biomaterialia · 2025Article
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- Galectin-3/Gelatin Electrospun Scaffolds Modulate Collagen Synthesis in Skin Healing but Do Not Improve Wound Closure Kinetics.Bioengineering (Basel, Switzerland) · 2024Article
- Fabrication of Porous Collagen Scaffolds Containing Embedded Channels with Collagen Membrane Linings.Micromachines · 2024Article
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- Direction-oriented fiber guiding with a tunable tri-layer-3D scaffold for periodontal regeneration.RSC advances · 2024Article
- Cell-homing and immunomodulatory composite hydrogels for effective wound healing with neovascularization.Bioactive materials · 2024Article
- The Influence of Various Crosslinking Conditions of EDC/NHS on the Properties of Fish Collagen Film.Marine drugs · 2024Article
- Water-Insoluble, Thermostable, Crosslinked Gelatin Matrix for Soft Tissue Implant Development.International journal of molecular sciences · 2024Article
- Covalent crosslinking chemistry for controlled modulation of nanometric roughness and surface free energy.Chemical science · 2024Article
- A biomimetic approach to modulating the sustained release of fibroblast growth factor 2 from fibrin microthread scaffolds.Exploration of biomat-x · 2024Article
- Effect of Electrical Stimulation on PC12 Cells Cultured in Different Hydrogels: Basis for the Development of Biomaterials in Peripheral Nerve Tissue Engineering.Pharmaceutics · 2023Article
10 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
This study focuses on determining the effect of varying the composition and crosslinking of collagen-based films on their physical properties and interaction with myoblasts. Films composed of collagen or gelatin and crosslinked with a carbodiimide were assessed for their surface roughness and stiffness. These samples are significant because they allow variation of physical properties as well as offering different recognition motifs for cell binding. Cell reactivity was determined by the ability of myoblastic C2C12 and C2C12-α2+ cell lines (with different integrin expression) to adhere to and spread on the films. Significantly, crosslinking reduced the cell reactivity of all films, irrespective of their initial composition, stiffness or roughness. Crosslinking resulted in a dramatic increase in the stiffness of the collagen film and also tended to reduce the roughness of the films (R(q) = 0.417 ± 0.035 μm, E = 31 ± 4.4 MPa). Gelatin films were generally smoother and more compliant than comparable collagen films (R(q) = 7.9 ± 1.5 nm, E = 15 ± 3.1 MPa). The adhesion of α2-positive cells was enhanced relative to the parental C2C12 cells on collagen compared with gelatin films. These results indicate that the detrimental effect of crosslinking on cell response may be due to the altered physical properties of the films as well as a reduction in the number of available cell binding sites. Hence, although crosslinking can be used to enhance the mechanical stiffness and reduce the roughness of films, it reduces their capacity to support cell activity and could potentially limit the effectiveness of the collagen-based films and scaffolds.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.