ArticleGene2012
Analyses of chromosome copy number and expression level of four genes in the ciliate Chilodonella uncinata reveal a complex pattern that suggests epigenetic regulation.
Article in Gene, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Epigenetics as Driver of Adaptation and Diversification in Microbial Eukaryotes.Frontiers in genetics · 2021Article
- Article
- Evolutionary origins and impacts of genome architecture in ciliates.Annals of the New York Academy of Sciences · 2019Review
- Twisted Tales: Insights into Genome Diversity of Ciliates Using Single-Cell 'Omics.Genome biology and evolution · 2018Article
- Article
- Insights into transgenerational epigenetics from studies of ciliates.European journal of protistology · 2017Review
- Nuclear Architecture and Patterns of Molecular Evolution Are Correlated in the Ciliate Chilodonella uncinata.Genome biology and evolution · 2016Article
- Article
- Genome structure drives patterns of gene family evolution in ciliates, a case study using Chilodonella uncinata (Protista, Ciliophora, Phyllopharyngea).Evolution; international journal of organic evolution · 2014Article
- Article
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Authors and funding
2 authors.
Funding
Abstract
Chilodonella uncinata, like all ciliates, contains two distinct nuclei in every cell: a germline micronucleus and a somatic macronucleus. The macronucleus develops from the zygotic nucleus through a series of chromosomal rearrangements. Macronuclear development in C. uncinata yields a nucleus with highly amplified gene-sized chromosomes. The macronucleus is transcriptionally active during vegetative growth while there is no expression in the micronucleus except during a brief period following conjugation. Gene family evolution in ciliates occurs through complex processes including gene duplication and an alternative processing of scrambled genes. Here we use quantitative PCR to compare relative expression levels of eight genes (SSU-rDNA, actin, α-tubulin and five β-tubulin sequences) to their abundance as macronuclear chromosomes. We show that three strains of the morphospecies C. uncinata share similar patterns across all loci. For example, we find an inverse correlation among five β-tubulin genes whereby the more abundant macronuclear chromosomes have lower levels of expression compared to less abundant chromosomes. We discuss the implication of our findings, which suggest that epigenetic mechanisms maintain chromosome copy number in C. uncinata.
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