ArticlePloS one2012
Control of germline stem cell division frequency--a novel, developmentally regulated role for epidermal growth factor signaling.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 47 citations in OpenAlex.
- Signaling pathways in Drosophila testis niche: Local signals that regulate stem cell fate.Insect science · 2026Review
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- Cell type- and chromosome-specific chromatin landscapes and DNA replication programs ofbioRxiv : the preprint server for biology · 2025Article
- Article
- Pvr regulates cyst stem cell division in the Drosophila testis niche, and has functions distinct from Egfr.Cells & development · 2023Article
- Formation of benign tumors by stem cell deregulation.PLoS genetics · 2022Review
- Article
- Article
- Epigenetic regulation of drosophila germline stem cell maintenance and differentiation.Developmental biology · 2021Review
- Single-cyst transcriptome analysis ofDevelopment (Cambridge, England) · 2020Article
- G-protein signaling is required for increasing germline stem cell division frequency in response to mating in Drosophila males.Scientific reports · 2020Article
- Receptor Tyrosine Kinases in Development: Insights fromInternational journal of molecular sciences · 2019Review
- Notch and Delta are required for survival of the germline stem cell lineage in testes of Drosophila melanogaster.PloS one · 2019Article
- Loss of heparan sulfate in the niche leads to tumor-like germ cell growth in the Drosophila testis.Glycobiology · 2018Article
- A study of the transit amplification divisions during spermatogenesis inEcology and evolution · 2018Article
- Somatic ERK activation during transit amplification is essential for maintaining the synchrony of germline divisions inOpen biology · 2018Article
- Protecting and Diversifying the Germline.Genetics · 2018Review
- Aubergine and piRNAs promote germline stem cell self-renewal by repressing the proto-oncogeneThe EMBO journal · 2017Article
- Polycomb Group Gene E(z) Is Required for Spermatogonial Dedifferentiation in Drosophila Adult Testis.Journal of molecular biology · 2017Article
- Enhancer of polycomb coordinates multiple signaling pathways to promote both cyst and germline stem cell differentiation in the Drosophila adult testis.PLoS genetics · 2017Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Exploring adult stem cell dynamics in normal and disease states is crucial to both better understanding their in vivo role and better realizing their therapeutic potential. Here we address the division frequency of Germline Stem Cells (GSCs) in testes of Drosophila melanogaster. We show that GSC division frequency is under genetic control of the highly conserved Epidermal Growth Factor (EGF) signaling pathway. When EGF signaling was attenuated, we detected a two-fold increase in the percentage of GSCs in mitotic division compared to GSCs in control animals. Ex vivo and in vivo experiments using a marker for cells in S-phase of the cell cycle showed that the GSCs in EGF mutant testes divide faster than GSCs in control testes. The increased mitotic activity of GSCs in EGF mutants was rescued by restoring EGF signaling in the GSCs, and reproduced in testes from animals with soma-depleted EGF-Receptor (EGFR). Interestingly, EGF attenuation specifically increased the GSC division frequency in adult testes, but not in larval testes. Furthermore, GSCs in testes with tumors resulting from the perturbation of other conserved signaling pathways divided at normal frequencies. We conclude that EGF signaling from the GSCs to the CySCs normally regulates GSC division frequency. The EGF signaling pathway is bifurcated and acts differently in adult compared to larval testes. In addition, regulation of GSC division frequency is a specific role for EGF signaling as it is not affected in all tumor models. These data advance our understanding concerning stem cell dynamics in normal tissues and in a tumor model.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.