ArticleProceedings of the National Academy of Sciences of the United States of America2012
Real-time analysis and selection of methylated DNA by fluorescence-activated single molecule sorting in a nanofluidic channel.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed.
- Epigenetic Control of Cold Stress Tolerance in Plants: Emerging Mechanisms and Applications for Crop Improvement.International journal of molecular sciences · 2026Review
- Quantitative characterization of liquids flowing in geometrically controlled sub-100 nm nanofluidic channels.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2023Article
- A Critical Review on the Sensing, Control, and Manipulation of Single Molecules on Optofluidic Devices.Micromachines · 2022Review
- Current and Emerging Technologies for the Analysis of the Genome-Wide and Locus-Specific DNA Methylation Patterns.Advances in experimental medicine and biology · 2022Article
- A Simple Low-Temperature Glass Bonding Process with Surface Activation by Oxygen Plasma for Micro/Nanofluidic Devices.Micromachines · 2020Article
- Microfluidic epigenomic mapping technologies for precision medicine.Lab on a chip · 2019Review
- Real-Time Selective Sequencing with RUBRIC: Read Until with Basecall and Reference-Informed Criteria.Scientific reports · 2019Article
- Analytical epigenetics: single-molecule optical detection of DNA and histone modifications.Current opinion in biotechnology · 2019Review
- Single-molecule DNA-mapping and whole-genome sequencing of individual cells.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Analysis of single nucleic acid molecules in micro- and nano-fluidics.Lab on a chip · 2016Review
- Article
- Characterization and directed evolution of a methyl-binding domain protein for high-sensitivity DNA methylation analysis.Protein engineering, design & selection : PEDS · 2015Article
- Single molecule and single cell epigenomics.Methods (San Diego, Calif.) · 2015Review
- Neuroepigenomics: Resources, Obstacles, and Opportunities.Neuroepigenetics · 2015Article
- Advances in the profiling of DNA modifications: cytosine methylation and beyond.Nature reviews. Genetics · 2014Review
- Developing epigenetic diagnostics and therapeutics for brain disorders.Trends in molecular medicine · 2013Review
- Epigenomics: one molecule at a time.Cell cycle (Georgetown, Tex.) · 2013Article
- Micro- and nanoscale devices for the investigation of epigenetics and chromatin dynamics.Nature nanotechnology · 2013Review
- Micro- and nanofluidic technologies for epigenetic profiling.Biomicrofluidics · 2013Article
- Single-molecule analysis of combinatorial epigenomic states in normal and tumor cells.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
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11 authors.
Funding
Abstract
Epigenetic modifications, such as DNA and histone methylation, are responsible for regulatory pathways that affect disease. Current epigenetic analyses use bisulfite conversion to identify DNA methylation and chromatin immunoprecipitation to collect molecules bearing a specific histone modification. In this work, we present a proof-of-principle demonstration for a new method using a nanofluidic device that combines real-time detection and automated sorting of individual molecules based on their epigenetic state. This device evaluates the fluorescence from labeled epigenetic modifications to actuate sorting. This technology has demonstrated up to 98% accuracy in molecule sorting and has achieved postsorting sample recovery on femtogram quantities of genetic material. We have applied it to sort methylated DNA molecules using simultaneous, multicolor fluorescence to identify methyl binding domain protein-1 (MBD1) bound to full-duplex DNA. The functionality enabled by this nanofluidic platform now provides a workflow for color-multiplexed detection, sorting, and recovery of single molecules toward subsequent DNA sequencing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.