ArticleAntimicrobial agents and chemotherapy2012
Effects of antimicrobial peptides on methanogenic archaea.
Article in Antimicrobial agents and chemotherapy, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 38 citations in OpenAlex.
- Emerging microbiome-directed therapies in inflammatory bowel disease: beyond diet modification and FMT.Seminars in immunopathology · 2025Review
- Development and characterisation of brown seaweed hydrolysates and fermentates with potential to reduce enteric methane.Scientific reports · 2025Article
- Exploring the human archaeome: its relevance for health and disease, and its complex interplay with the human immune system.The FEBS journal · 2025Review
- The effect ofMicrobiology spectrum · 2024Article
- Review
- The evolving role of methanogenic archaea in mammalian microbiomes.Frontiers in microbiology · 2023Review
- The Historical Development of Cultivation Techniques for Methanogens and Other Strict Anaerobes and Their Application in Modern Microbiology.Microorganisms · 2022Review
- Antimicrobial Peptides Controlling Resistant Bacteria in Animal Production.Frontiers in microbiology · 2022Review
- Histone variants in archaea and the evolution of combinatorial chromatin complexity.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Seaweed and Seaweed Bioactives for Mitigation of Enteric Methane: Challenges and Opportunities.Animals : an open access journal from MDPI · 2020Review
- The host-associated archaeome.Nature reviews. Microbiology · 2020Review
- Archaeosine Modification of Archaeal tRNA: Role in Structural Stabilization.Journal of bacteriology · 2020Article
- Membrane Lipid Composition and Amino Acid Excretion Patterns ofLife (Basel, Switzerland) · 2019Article
- Use of Lactic Acid Bacteria to Reduce Methane Production in Ruminants, a Critical Review.Frontiers in microbiology · 2019Review
- Development of Multiwell-Plate Methods Using Pure Cultures of Methanogens To Identify New Inhibitors for Suppressing Ruminant Methane Emissions.Applied and environmental microbiology · 2017Article
- Article
- The Human-Associated ArchaeonFrontiers in immunology · 2017Article
- Biofilm formation of mucosa-associated methanoarchaeal strains.Frontiers in microbiology · 2014Article
- The intestinal archaea Methanosphaera stadtmanae and Methanobrevibacter smithii activate human dendritic cells.PloS one · 2014Article
- Assessment of the oxidant tolerance of Methanosarcina acetivorans.FEMS microbiology letters · 2013Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As members of the indigenous human microbiota found on several mucosal tissues, Methanobrevibacter smithii and Methanosphaera stadtmanae are exposed to the effects of antimicrobial peptides (AMPs) secreted by these epithelia. Although antimicrobial and molecular effects of AMPs on bacteria are well described, data for archaea are not available yet. Besides, it is not clear whether AMPs affect them as the archaeal cell envelope differs profoundly in terms of chemical composition and structure from that of bacteria. The effects of different synthetic AMPs on growth of M. smithii, M. stadtmanae, and Methanosarcina mazei were tested using a microtiter plate assay adapted to their anaerobic growth requirements. All three tested methanoarchaea were highly sensitive against derivatives of human cathelicidin, of porcine lysin, and a synthetic antilipopolysaccharide peptide (Lpep); however, sensitivities differed markedly among the methanoarchaeal strains. The potent AMP concentrations affecting growth were below 10 μM, whereas growth of Escherichia coli WBB01 was not affected at peptide concentrations up to 10 μM under the same anaerobic growth conditions. Atomic force microscopy and transmission electron microscopy revealed that the structural integrity of the methanoarchaeal cells is destroyed within 4 h after incubation with AMPs. The disruption of the cell envelope of M. smithii, M. stadtmanae, and M. mazei within a few minutes of exposure was verified by using LIVE/DEAD staining. Our results strongly suggest that the release of AMPs by eukaryotic epithelial cells is a potent defense mechanism targeting not only bacteria, but also methanoarchaea.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.