ArticleThe Journal of biological chemistry2012
Balancing Arc synthesis, mRNA decay, and proteasomal degradation: maximal protein expression triggered by rapid eye movement sleep-like bursts of muscarinic cholinergic receptor stimulation.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 74 citations in OpenAlex.
- Distinct Molecular Responses to Ketamine and Imipramine in Cortical and Striatal Regions Following Acute Swim Stress.Biomolecules · 2026Article
- Arc Expression in the Dentate Gyrus of F344 Rats Across the Sleep-Wake Cycle.microPublication biology · 2026Article
- CYFIP1 governs the development of cortical axons by modulating calcium availability.Nature communications · 2025Article
- Cognitive outcomes of deep brain stimulation depend on age and hippocampal connectivity in Parkinson's and Alzheimer's disease.Alzheimer's & dementia : the journal of the Alzheimer's Association · 2025Article
- DELTA: a method for brain-wide measurement of synaptic protein turnover reveals localized plasticity during learning.Nature neuroscience · 2025Article
- A Nanobody-Based Proximity Ligation Assay Detects Constitutive and Stimulus-Regulated Native Arc/Arg3.1 Oligomers in Hippocampal Neuronal Dendrites.Molecular neurobiology · 2025Article
- Dopamine D1-Like Receptor Stimulation Induces CREB, Arc, and BDNF Dynamic Changes in Differentiated SH-SY5Y Cells.Neurochemical research · 2024Article
- Overnight neuronal plasticity and adaptation to emotional distress.Nature reviews. Neuroscience · 2024Review
- Structural characterization of two nanobodies targeting the ligand-binding pocket of human Arc.PloS one · 2024Article
- Article
- Detection of Arc/Arg3.1 oligomers in rat brain: constitutive and synaptic activity-evoked dimer expressionFrontiers in molecular neuroscience · 2023Article
- Molecular physiology of Arc/Arg3.1: The oligomeric state hypothesis of synaptic plasticity.Acta physiologica (Oxford, England) · 2022Review
- Development and Validation of Arc Nanobodies: New Tools for Probing Arc Dynamics and Function.Neurochemical research · 2022Article
- 'Arc'-hitecture of normal cognitive aging.Ageing research reviews · 2022Review
- Translational changes induced by acute sleep deprivation uncovered by TRAP-Seq.Molecular brain · 2020Article
- UPF2 leads to degradation of dendritically targeted mRNAs to regulate synaptic plasticity and cognitive function.Molecular psychiatry · 2020Article
- ATP11B deficiency leads to impairment of hippocampal synaptic plasticity.Journal of molecular cell biology · 2019Article
- Activity-Regulated Cytoskeleton-Associated Protein (Arc/Arg3.1) is Transiently Expressed after Heat Shock Stress and Suppresses Heat Shock Factor 1.Scientific reports · 2019Article
- The Arc of cognition: Signaling cascades regulating Arc and implications for cognitive function and disease.Seminars in cell & developmental biology · 2018Review
- Herpes Simplex Virus Type 1 Enhances Expression of the Synaptic Protein Arc for Its Own Benefit.Frontiers in cellular neuroscience · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cholinergic signaling induces Arc/Arg3.1, an immediate early gene crucial for synaptic plasticity. However, the molecular mechanisms that dictate Arc mRNA and protein dynamics during and after cholinergic epochs are little understood. Using human SH-SY5Y neuroblastoma cells, we show that muscarinic cholinergic receptor (mAchR) stimulation triggers Arc synthesis, whereas translation-dependent RNA decay and proteasomal degradation strictly limit the amount and duration of Arc expression. Chronic application of the mAchR agonist, carbachol (Cch), induces Arc transcription via ERK signaling and release of calcium from IP(3)-sensitive stores. Arc translation requires ERK activation, but not changes in intracellular calcium. Proteasomal degradation of Arc (half-life ∼37 min) was enhanced by thapsigargin, an inhibitor of the endoplasmic calcium-ATPase pump. Similar mechanisms of Arc protein regulation were observed in cultured rat hippocampal slices. Functionally, we studied the impact of cholinergic epoch duration and temporal pattern on Arc protein expression. Acute Cch treatment (as short as 2 min) induces transient, moderate Arc expression, whereas continuous treatment of more than 30 min induces maximal expression, followed by rapid decline. Cholinergic activity associated with rapid eye movement sleep may function to facilitate long term synaptic plasticity and memory. Employing a paradigm designed to mimic intermittent rapid eye movement sleep epochs, we show that application of Cch in a series of short bursts generates persistent and maximal Arc protein expression. The results demonstrate dynamic, multifaceted control of Arc synthesis during mAchR signaling, and implicate cholinergic epoch duration and repetition as critical determinants of Arc expression and function in synaptic plasticity and behavior.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.