ArticleBMC plant biology2012
Development of high amylose wheat through TILLING.
Article in BMC plant biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
78 citing papers in PubMed, 227 citations in OpenAlex.
- Resistant Starch Type 2 from Wheat Reduces Postprandial Glycemic Response with Concurrent Alterations in Gut Microbiota Composition.Nutrients · 2021Trial
- High-Amylose Starch and Human Health: Microbiota Modulation, Metabolic Reprogramming, and Disease Prevention.Nutrients · 2026Review
- Advances in gene cloning and functional genomics approaches for wheat (Triticum aestivum L.) improvement.The plant genome · 2026Review
- Altered starch composition and improved bread quality in novel wheat mutant reveal key transcriptional regulators.Current research in food science · 2026Article
- Food as Medicine: Curbing Type-2 Diabetes Prevalence Through Consumption of High Amylose Starchy Foods in Sub-Saharan Africa.Advanced pharmaceutical bulletin · 2025Review
- Wheat improvement through advances in single nucleotide polymorphism (SNP) detection and genotyping with a special emphasis on rust resistance.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Review
- Genome-wide association study identifies loci and candidate genes for RVA parameters in wheat (Frontiers in plant science · 2024Article
- Impact of Variation in Amylose Content on Durum Wheat cv. Svevo Technological and Starch Properties.Foods (Basel, Switzerland) · 2023Article
- Snapshot of four mature quinoa (Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- Effect of Three Bakery Products Formulated with High-Amylose Wheat Flour on Post-Prandial Glycaemia in Healthy Volunteers.Foods (Basel, Switzerland) · 2023Article
- Genetic insights in pearl millet breeding in the genomic era: challenges and prospects.Plant biotechnology reports · 2023Review
- Functional analysis of polyphenol oxidase 1 gene in common wheat.Frontiers in plant science · 2023Article
- Is it the end of TILLING era in plant science?Frontiers in plant science · 2023Review
- Phytochemical Profiling and Untargeted Metabolite Fingerprinting of the MEDWHEALTH Wheat, Barley and Lentil Wholemeal Flours.Foods (Basel, Switzerland) · 2022Article
- Environmental Profile of a Novel High-Amylose Bread Wheat Fresh Pasta with Low Glycemic Index.Foods (Basel, Switzerland) · 2022Article
- Characterization of Fresh Pasta Made of Common and High-Amylose Wheat Flour Mixtures.Foods (Basel, Switzerland) · 2022Article
- Loss of starch synthase IIIa changes starch molecular structure and granule morphology in grains of hexaploid bread wheat.Scientific reports · 2022Article
- Unraveling novel and rare mutations for alpha-amylase and key transcription factors in EMS-induced wheat mutants for amylose by TILLING.Molecular biology reports · 2022Article
- Reverse genetic approaches for breeding nutrient-rich and climate-resilient cereal and food legume crops.Heredity · 2022Article
- Increasing the Versatility of Durum Wheat through Modifications of Protein and Starch Composition and Grain Hardness.Foods (Basel, Switzerland) · 2022Review
18 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
Abstract
backgroundWheat (Triticum spp.) is an important source of food worldwide and the focus of considerable efforts to identify new combinations of genetic diversity for crop improvement. In particular, wheat starch composition is a major target for changes that could benefit human health. Starches with increased levels of amylose are of interest because of the correlation between higher amylose content and elevated levels of resistant starch, which has been shown to have beneficial effects on health for combating obesity and diabetes. TILLING (Targeting Induced Local Lesions in Genomes) is a means to identify novel genetic variation without the need for direct selection of phenotypes.
resultsUsing TILLING to identify novel genetic variation in each of the A and B genomes in tetraploid durum wheat and the A, B and D genomes in hexaploid bread wheat, we have identified mutations in the form of single nucleotide polymorphisms (SNPs) in starch branching enzyme IIa genes (SBEIIa). Combining these new alleles of SBEIIa through breeding resulted in the development of high amylose durum and bread wheat varieties containing 47-55% amylose and having elevated resistant starch levels compared to wild-type wheat. High amylose lines also had reduced expression of SBEIIa RNA, changes in starch granule morphology and altered starch granule protein profiles as evaluated by mass spectrometry.
conclusionsWe report the use of TILLING to develop new traits in crops with complex genomes without the use of transgenic modifications. Combined mutations in SBEIIa in durum and bread wheat varieties resulted in lines with significantly increased amylose and resistant starch contents.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.