ArticleMicrobial cell factories2012
Expression of lignocellulolytic enzymes in Pichia pastoris.
Article in Microbial cell factories, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Reporter Group-Labeled Synthetic Cellulose: Structural Characterization and Utilization in Mapping the Cellulose Chain-Cleavage Modes of Cellulases.Biomacromolecules · 2026Article
- Strangers in a foreign land: 'Yeastizing' plant enzymes.Microbial biotechnology · 2024Review
- Profiling expression strategies for a type III polyketide synthase in a lysate-based, cell-free system.Scientific reports · 2024Article
- Unlocking the potential of feruloyl esterase from3 Biotech · 2024Article
- Scalable protein production by Komagataella phaffii enabled by ARS plasmids and carbon source-based selection.Microbial cell factories · 2024Article
- Burden Imposed by Heterologous Protein Production in Two Major Industrial Yeast Cell Factories: Identifying Sources and Mitigation Strategies.Frontiers in fungal biology · 2022Review
- Strains and Molecular Tools for Recombinant Protein Production in Pichia pastoris.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Customized yeast cell factories for biopharmaceuticals: from cell engineering to process scale up.Microbial cell factories · 2021Review
- Codon optimization with deep learning to enhance protein expression.Scientific reports · 2020Article
- Establishing a EukaryoticFrontiers in bioengineering and biotechnology · 2020Article
- Industrial Use of Cell Wall Degrading Enzymes: The Fine Line Between Production Strategy and Economic Feasibility.Frontiers in bioengineering and biotechnology · 2020Review
- Efficient Expression of Xylanase by Codon Optimization and Its Effects on the Growth Performance and Carcass Characteristics of Broiler.Animals : an open access journal from MDPI · 2019Article
- Single-Cell Approach to Monitor the Unfolded Protein Response During Biotechnological Processes WithFrontiers in microbiology · 2019Article
- Effect of Plasmid Design and Type of Integration Event on Recombinant Protein Expression in Pichia pastoris.Applied and environmental microbiology · 2018Article
- A Novel Vector for Construction of Markerless Multicopy Overexpression Transformants inFrontiers in microbiology · 2017Article
- Recombinant Expression of Trichoderma reesei Cel61A in Pichia pastoris: Optimizing Yield and N-terminal Processing.Molecular biotechnology · 2015Article
- Construction of efficient xylose utilizing Pichia pastoris for industrial enzyme production.Microbial cell factories · 2015Article
- Cellulose surface degradation by a lytic polysaccharide monooxygenase and its effect on cellulase hydrolytic efficiency.The Journal of biological chemistry · 2014Article
- Expression of endoglucanases in Pichia pastoris under control of the GAP promoter.Microbial cell factories · 2014Article
- Expression of codon optimized genes in microbial systems: current industrial applications and perspectives.Frontiers in microbiology · 2014Review
Corrections and comments
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4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSustainable utilization of plant biomass as renewable source for fuels and chemical building blocks requires a complex mixture of diverse enzymes, including hydrolases which comprise the largest class of lignocellulolytic enzymes. These enzymes need to be available in large amounts at a low price to allow sustainable and economic biotechnological processes.Over the past years Pichia pastoris has become an attractive host for the cost-efficient production and engineering of heterologous (eukaryotic) proteins due to several advantages.
resultsIn this paper codon optimized genes and synthetic alcohol oxidase 1 promoter variants were used to generate Pichia pastoris strains which individually expressed cellobiohydrolase 1, cellobiohydrolase 2 and beta-mannanase from Trichoderma reesei and xylanase A from Thermomyces lanuginosus. For three of these enzymes we could develop strains capable of secreting gram quantities of enzyme per liter in fed-batch cultivations. Additionally, we compared our achieved yields of secreted enzymes and the corresponding activities to literature data.
conclusionIn our experiments we could clearly show the importance of gene optimization and strain characterization for successfully improving secretion levels. We also present a basic guideline how to correctly interpret the interplay of promoter strength and gene dosage for a successful improvement of the secretory production of lignocellulolytic enzymes in Pichia pastoris.
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