ArticleApplied and environmental microbiology2012
A straightforward DOPE (double labeling of oligonucleotide probes)-FISH (fluorescence in situ hybridization) method for simultaneous multicolor detection of six microbial populations.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Newly Designed Fluorescence In Situ Hybridization Probes Reveal Previously Unknown Endophytic Abilities of Tuber magnatum in Herbaceous Plants.Microbial ecology · 2025Article
- Imaging biofilms using fluorescenceFrontiers in cellular and infection microbiology · 2023Review
- Application of Fluorescence In Situ Hybridization (FISH) in Oral Microbial Detection.Pathogens (Basel, Switzerland) · 2022Review
- Microbes from Mum: symbiont transmission in the tropical reef sponge Ianthella basta.ISME communications · 2022Article
- Recent advances in tools to map the microbiome.Current opinion in biomedical engineering · 2021Article
- FISH Variants.Methods in molecular biology (Clifton, N.J.) · 2021Review
- NOTIFy (non-toxic lyophilized field)-FISH for the identification of biological agents by Fluorescence in situ Hybridization.PloS one · 2020Article
- A Multicolor FluorescenceFrontiers in microbiology · 2019Article
- Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization.Journal of visualized experiments : JoVE · 2018Article
- Organization of supragingival plaque at the micron scale.Journal of oral microbiology · 2018Article
- FluorescenceFrontiers in medicine · 2017Article
- NovelFrontiers in microbiology · 2017Article
- probeBase--an online resource for rRNA-targeted oligonucleotide probes and primers: new features 2016.Nucleic acids research · 2016Article
- MiL-FISH: Multilabeled Oligonucleotides for Fluorescence In Situ Hybridization Improve Visualization of Bacterial Cells.Applied and environmental microbiology · 2016Article
- New Technologies for Studying Biofilms.Microbiology spectrum · 2015Review
- A flow cytometric approach to quantify biofilms.Folia microbiologica · 2015Article
- In situ visualization of newly synthesized proteins in environmental microbes using amino acid tagging and click chemistry.Environmental microbiology · 2014Article
- Whole cell hybridisation for monitoring harmful marine microalgae.Environmental science and pollution research international · 2013Article
- New methods for analysis of spatial distribution and coaggregation of microbial populations in complex biofilms.Applied and environmental microbiology · 2013Article
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4 authors.
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Abstract
Fluorescence in situ hybridization (FISH) with rRNA-targeted oligonucleotide probes is an essential tool for the cultivation-independent identification of microbes within environmental and clinical samples. However, one of the major constraints of conventional FISH is the very limited number of different target organisms that can be detected simultaneously with standard epifluorescence or confocal laser scanning microscopy. Recently, this limitation has been overcome via an elegant approach termed combinatorial labeling and spectral imaging FISH (CLASI-FISH) (23). This technique, however, suffers compared to conventional FISH from an inherent loss in sensitivity and potential probe binding biases caused by the competition of two differentially labeled oligonucleotide probes for the same target site. Here we demonstrate that the application of multicolored, double-labeled oligonucleotide probes enables the simultaneous detection of up to six microbial target populations in a straightforward and robust manner with higher sensitivity and less bias. Thus, this newly developed technique should be an attractive option for all researchers interested in applying conventional FISH methods for the study of microbial communities.
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