ArticleApplied and environmental microbiology2012
Sensitive quantification of Clostridium difficile cells by reverse transcription-quantitative PCR targeting rRNA molecules.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 47 citations in OpenAlex.
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- Association between postoperative changes in the gut microbiota and pseudopsia after cardiac surgery: prospective observational study.BMC surgery · 2020Observational
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- Detection of bacteria in blood circulation in patients receiving cancer chemotherapy.International journal of clinical oncology · 2020Article
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- Single enzyme RT-PCR of full-length ribosomal RNA.Synthetic biology (Oxford, England) · 2020Article
- Gut microbiota profile in healthy Indonesians.World journal of gastroenterology · 2019Article
- Independent and Interactive Effects of Habitually Ingesting Fermented Milk Products ContainingFrontiers in microbiology · 2019Article
- Gut Microbiota Composition Before and After Use of Proton Pump Inhibitors.Digestive diseases and sciences · 2018Observational
- A low-cost paper-based synthetic biology platform for analyzing gut microbiota and host biomarkers.Nature communications · 2018Article
- Counting the Countless: Bacterial Quantification by Targeting rRNA Molecules to Explore the Human Gut Microbiota in Health and Disease.Frontiers in microbiology · 2018Review
- Evolution of gut Bifidobacterium population in healthy Japanese infants over the first three years of life: a quantitative assessment.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We established a sensitive and accurate quantification system for Clostridium difficile in human intestines, based on rRNA-targeted reverse transcription-quantitative PCR (RT-qPCR). We newly developed a species-specific primer set for C. difficile targeting 23S rRNA gene sequences. Both the vegetative cells and the spores of C. difficile in human feces were quantified by RT-qPCR, with a lower detection limit of 10(2.4) cells/g of feces. In an analysis of the feces of residents (n = 83; age, 85 ± 8 years) and staff (n = 19; age, 36 ± 10 years) at a care facility for the elderly, C. difficile was detected by RT-qPCR in 43% of the residents (average count, log(10) 4.0 ± 2.0 cells/g of feces) and 16% of the staff (average count, log(10) 2.2 ± 0.1 cells/g of feces); these rates were far higher than those detected by qPCR (residents, 19%; staff, 0%) or selective cultivation (residents, 18%; staff, 5%). Another analysis of healthy adults (n = 63; age, 41 ± 11 years) also revealed the significant carriage rate of C. difficile in the intestines (detection rate, 13%; average count, log(10) 4.9 ± 1.2 cells/g of feces). From these results, it was suggested that rRNA-targeted RT-qPCR should be an effective tool for analyzing population levels of C. difficile in the human intestine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.