ArticleApplied and environmental microbiology2012
Natural transformation of Gallibacterium anatis.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed.
- The absence ofFrontiers in veterinary science · 2024Article
- Gallibacterium anatis infection in poultry: a comprehensive review.Tropical animal health and production · 2023Review
- Isolation and characterization of multidrug resistant Gallibacterium anatis biovar haemolytica strains from Polish geese and hens.Veterinary research · 2023Article
- Basic Characterization of Natural Transformation in Avibacterium paragallinarum.Microbiology spectrum · 2023Article
- Genetic Organization of Acquired Antimicrobial Resistance Genes and Detection of Resistance-Mediating Mutations in aAntibiotics (Basel, Switzerland) · 2023Article
- A putative siderophore receptor ofFrontiers in microbiology · 2022Article
- Natural Transformation as a Mechanism of Horizontal Gene Transfer inPathogens (Basel, Switzerland) · 2021Article
- Effect of Nutritional Determinants and TonB on the Natural Transformation ofFrontiers in microbiology · 2021Article
- Isolation of Drug-Resistant Gallibacterium anatis from Calves with Unresponsive Bronchopneumonia, Belgium.Emerging infectious diseases · 2020Article
- BlaCurrent microbiology · 2019Article
- DprA Is Essential for Natural Competence inFrontiers in genetics · 2019Article
- Use of Natural Transformation To Establish an Easy Knockout Method in Riemerella anatipestifer.Applied and environmental microbiology · 2017Article
- Efficient construction ofCanadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2016Article
- Dissemination of Antimicrobial Resistance in Microbial Ecosystems through Horizontal Gene Transfer.Frontiers in microbiology · 2016Review
- Bacterial determinants of importance in the virulence of Gallibacterium anatis in poultry.Veterinary research · 2015Review
- Natural competence and the evolution of DNA uptake specificity.Journal of bacteriology · 2014Review
- Horizontal gene transfer in the human gastrointestinal tract: potential spread of antibiotic resistance genes.Infection and drug resistance · 2014Review
- Draft Genome Sequence of Gallibacterium anatis bv. haemolytica 12656-12 Liver, an Isolate Obtained from the Liver of a Septicemic Chicken.Genome announcements · 2013Article
- The fimbrial protein FlfA from Gallibacterium anatis is a virulence factor and vaccine candidate.Infection and immunity · 2013Article
- The availability of purine nucleotides regulates natural competence by controlling translation of the competence activator Sxy.Molecular microbiology · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gallibacterium anatis is a pathogen of poultry. Very little is known about its genetics and pathogenesis. To enable the study of gene function in G. anatis, we have established methods for transformation and targeted mutagenesis. The genus Gallibacterium belongs to the Pasteurellaceae, a group with several naturally transformable members, including Haemophilus influenzae. Bioinformatics analysis identified G. anatis homologs of the H. influenzae competence genes, and natural competence was induced in G. anatis by the procedure established for H. influenzae: transfer from rich medium to the starvation medium M-IV. This procedure gave reproducibly high transformation frequencies with G. anatis chromosomal DNA and with linearized plasmid DNA carrying G. anatis sequences. Both DNA types integrated into the G. anatis chromosome by homologous recombination. Targeted mutagenesis gave transformation frequencies of >2 × 10(-4) transformants CFU(-1). Transformation was also efficient with circular plasmid containing no G. anatis DNA; this resulted in the establishment of a self-replicating plasmid. Nine diverse G. anatis strains were found to be naturally transformable by this procedure, suggesting that natural competence is common and the M-IV transformation procedure widely applicable for this species. The G. anatis genome is only slightly enriched for the uptake signal sequences identified in other pasteurellaceaen genomes, but G. anatis did preferentially take up its own DNA over that of Escherichia coli. Transformation by electroporation was not effective for chromosomal integration but could be used to introduce self-replicating plasmids. The findings described here provide important tools for the genetic manipulation of G. anatis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.