ArticleGlycobiology2012
Detailed O-glycomics of the Muc2 mucin from colon of wild-type, core 1- and core 3-transferase-deficient mice highlights differences compared with human MUC2.
Article in Glycobiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
67 citing papers in PubMed, 100 citations in OpenAlex.
- Fast structural search for classification of gut bacterial mucin O-glycan degrading enzymes.PLoS computational biology · 2026Article
- Glycosphingolipids in human abdominal leiomyosarcoma and liposarcoma.The Journal of biological chemistry · 2026Article
- Degradation of mucinbioRxiv : the preprint server for biology · 2026Article
- MUC2 shifts the metabolic profiles of commensal bacteria in defined microbial communities.Microbiome research reports · 2026Article
- Mucinase fromFrontiers in microbiology · 2026Article
- Using MUC2 mucin producing tumorigenic human goblet-like cells to uncover functional properties of the mucus barrier.Gut microbes · 2025Article
- New insights in amino sugar metabolism by the gut microbiome.Gut microbes · 2025Review
- Intestinal Mucin Glycosylation: Structural Regulation, Homeostasis Maintenance and Disease Association.Biomolecules · 2025Review
- Tools for investigating host-microbe crosstalk using glycan analysis probes inspired by human lectins.Glycobiology · 2025Article
- A glycan atlas of the mammalian intestine through ontogeny and inflammation.bioRxiv : the preprint server for biology · 2025Article
- Deep crypt secretory cells shape region-specific mucin glycosylation patterns in the mouse colon.PloS one · 2025Article
- Article
- Glycan analysis probes inspired by human lectins for investigating host-microbe crosstalk.bioRxiv : the preprint server for biology · 2024Article
- O-glycosylation contributes to mammalian glycoRNA biogenesis.bioRxiv : the preprint server for biology · 2024Article
- The evolutionarily ancient FOXA transcription factors shape the murine gut microbiome via control of epithelial glycosylation.Developmental cell · 2024Article
- Article
- Revealing Glycosylation Patterns inJournal of agricultural and food chemistry · 2024Article
- Article
- Role of mucin glycosylation in the gut microbiota-brain axis of core 3 O-glycan deficient mice.Scientific reports · 2023Article
- Intestinal mucus and their glycans: A habitat for thriving microbiota.Cell host & microbe · 2023Review
7 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
The heavily O-glycosylated mucin MUC2 constitutes the major protein in the mucosal layer that acts as a physical barrier protecting the epithelial layer in the colon. In this study, Muc2 was purified from mucosal scrapings from the colon of wild-type (WT) mice, core 3 transferase knockout (C3Gnt(-/-)) mice and intestinal epithelial cell-specific core 1 knockout (IEC C1Galt1(-/-)) mice. The Muc2 O-glycans were released by reductive β-elimination and analyzed with liquid chromatography-mass spectrometry in the negative-ion mode. Muc2 from the distal colon of WT and C3Gnt(-/-) knockout mice carried a mixture of core 1- or core 2-type glycans, whereas Muc2 from IEC C1Galt1(-/-) mice carried highly sialylated core 3- and core 4-type glycans. A large portion of NeuAc in all mouse models was positioned on disialylated N-acetyllactosamine units, an epitope not reported on human colonic MUC2. Mass spectra and proton NMR spectroscopy revealed an abundant NeuAc linked to internally positioned N-acetylglucosamine on colonic murine Muc2, which also differs markedly from human MUC2. Our results highlight that murine colonic Muc2 O-glycosylation is substantially different from human MUC2, which could be one explanation for the different commensal microbiota of these two species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.