ArticleNucleic acids research2012
Topological characterization of the DnaA-oriC complex using single-molecule nanomanipuation.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 27 citations in OpenAlex.
- Mechanisms of chromosomal DNA replication in Escherichia coli and Bacillus subtilis.FEMS microbiology reviews · 2026Review
- Review
- Open Questions about the Roles of DnaA, Related Proteins, and Hyperstructure Dynamics in the Cell Cycle.Life (Basel, Switzerland) · 2023Review
- Article
- Interactions of replication initiator RctB with single- and double-stranded DNA in origin opening of Vibrio cholerae chromosome 2.Nucleic acids research · 2020Article
- Dynamics of DNA nicking and unwinding by the RepC-PcrA complex.Nucleic acids research · 2020Article
- Identification of the Unwinding Region in theFrontiers in microbiology · 2020Article
- Origins of DNA replication.PLoS genetics · 2019Review
- Identification of a basal system for unwinding a bacterial chromosome origin.The EMBO journal · 2019Article
- The plasmid-borne quinolone resistance protein QnrB, a novel DnaA-binding protein, increases the bacterial mutation rate by triggering DNA replication stress.Molecular microbiology · 2019Article
- Changing Perspectives on the Role of DnaA-ATP in Orisome Function and Timing Regulation.Frontiers in microbiology · 2019Review
- Origin recognition is the predominant role for DnaA-ATP in initiation of chromosome replication.Nucleic acids research · 2018Article
- Review
- Opening the Strands of Replication Origins-Still an Open Question.Frontiers in molecular biosciences · 2016Review
- The Arg Fingers of Key DnaA Protomers Are Oriented Inward within the Replication Origin oriC and Stimulate DnaA Subcomplexes in the Initiation Complex.The Journal of biological chemistry · 2015Article
- Bacterial initiators form dynamic filaments on single-stranded DNA monomer by monomer.Nucleic acids research · 2015Article
- The orisome: structure and function.Frontiers in microbiology · 2015Review
- Sequence-specific interactions of Rep proteins with ssDNA in the AT-rich region of the plasmid replication origin.Nucleic acids research · 2014Article
- oriC-encoded instructions for the initiation of bacterial chromosome replication.Frontiers in microbiology · 2014Review
- Bacterial replication, transcription and translation: mechanistic insights from single-molecule biochemical studies.Nature reviews. Microbiology · 2013Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In most bacteria, the timing and synchrony of initiation of chromosomal replication are determined by the binding of the AAA(+) protein DnaA to a set of high- and low-affinity sites found within the origin of chromosomal replication (oriC). Despite the large amount of information on the role and regulation of DnaA, the actual structure of the DnaA-oriC complex and the mechanism by which it primes the origin for the initiation of replication remain unclear. In this study, we have performed magnetic tweezers experiments to investigate the structural properties of the DnaA-oriC complex. We show that the DnaA-ATP-oriC complex adopts a right-handed helical conformation involving a variable amount of DNA and protein whose features fit qualitatively as well as quantitatively with an existing model based on the crystal structure of a truncated DnaA tetramer obtained in the absence of DNA. We also investigate the topological effect of oriC's DNA unwinding element.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.