ArticleThe EMBO journal2012
ARF6 GTPase protects the post-mitotic midbody from 14-3-3-mediated disintegration.
Article in The EMBO journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 42 citations in OpenAlex.
- A key role for centralspindlin and Ect2 in the development of multicellularity and the emergence of Metazoa.Current biology : CB · 2025Article
- ARF6, a component of intercellular bridges, is essential for spermatogenesis in mice.Developmental biology · 2024Article
- Mechanistic insights into central spindle assembly mediated by the centralspindlin complex.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- An ATM-Chk2-INCENP pathway activates the abscission checkpoint.The Journal of cell biology · 2021Article
- Inhibition of neddylation induces mitotic defects and alters MKLP1 accumulation at the midbody during cytokinesis.Cell cycle (Georgetown, Tex.) · 2019Article
- Cellular and subcellular localization of ADP-ribosylation factor 6 in mouse peripheral tissues.Histochemistry and cell biology · 2017Article
- Proteomics Screen Identifies Class I Rab11 Family Interacting Proteins as Key Regulators of Cytokinesis.Molecular and cellular biology · 2017Article
- MICAL3 Flavoprotein Monooxygenase Forms a Complex with Centralspindlin and Regulates Cytokinesis.The Journal of biological chemistry · 2016Article
- Class I Arfs (Arf1 and Arf3) and Arf6 are localized to the Flemming body and play important roles in cytokinesis.Journal of biochemistry · 2016Article
- CYK4 promotes antiparallel microtubule bundling by optimizing MKLP1 neck conformation.PLoS biology · 2015Article
- Phosphorylation of CHO1 by Lats1/2 regulates the centrosomal activation of LIMK1 during cytokinesis.Cell cycle (Georgetown, Tex.) · 2015Article
- Prime movers: the mechanochemistry of mitotic kinesins.Nature reviews. Molecular cell biology · 2014Review
- SHCBP1 is required for midbody organization and cytokinesis completion.Cell cycle (Georgetown, Tex.) · 2014Article
- An integrated overview of spatiotemporal organization and regulation in mitosis in terms of the proteins in the functional supercomplexes.Frontiers in microbiology · 2014Review
- The molecular basis for kinesin functional specificity during mitosis.Cytoskeleton (Hoboken, N.J.) · 2013Review
- Centralspindlin: at the heart of cytokinesis.Cytoskeleton (Hoboken, N.J.) · 2012Review
- Cytokinesis microtubule organisers at a glance.Journal of cell science · 2012Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
In cytokinesis, there is a lengthy interval between cleavage furrow ingression and abscission, during which the midbody microtubule bundle provides both structural support for a narrow intercellular bridge and a platform that orchestrates the biochemical preparations for abscission. It is currently unclear how the midbody structure is stably maintained during this period. Here, we report a novel role for the ADP-ribosylation factor 6 (ARF6) GTPase in the post-mitotic stabilisation of midbody. Centralspindlin kinesin-6/RhoGAP complex, a midbody component critical for both the formation and function of the midbody, assembles in a sharp band at the centre of the structure in a manner antagonised by 14-3-3 protein. We show that ARF6 competes with 14-3-3 for binding to centralspindlin such that midbodies formed by centralspindlin mutants that can bind 14-3-3 but not ARF6 frequently collapse before abscission. These data indicate a novel mechanism for the regulation of midbody dynamics in which ARF6 protects the compacted centralspindlin assembly from dissipation by 14-3-3.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.